XB-CLONE-3562282
Clone Name: IMAGE:7710622
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Gene:
hnrnpk
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967044 [+]
tataagttgtattaaaagaagatggagacagagcaggaagaggttgaattcactaacacagaaactaacggtaaacgcccagcagaagacatggaggagg
aacaggcatttaagagatctagaaacacagatatgatggttgagctgcgtattctgcttcagagcaagaatgctggagcagtaatcggcaaaggaggaaa
aaatattaaggcgcttaggacagactacaatgccagtgtttcagtcccagacagcagtggccccgagcgtatattaagcatcagcgcagatattgaaaca
attggagaaatcctaaagaagataattcccacactggaagagtaccaacacttcaaaggaaatgattttgattgcgagttgaggttattgattcatcaaa
gtttggctggtggaattattggtgtcaaaggagcaaagattaaagaacttagagagaaaactcagactacaataaagctatttcaggagtgctgccctca
ttctactgatcgtgttgtgctgattgggggaaaacctgacagggtggttgaatgcattaaagttatacttgatctcatctctgagtcccctgtaaagggc
aggtctcaaccctatgaccctaacttctatgacgaaacttatgattatggaggatttacaatgatgtttgatgaccgaagaggacgccccatgggttttc
ctatgcatgctagaggtggatttgatcgaatgcctccaggacccggtggtcgccccttgccacagtcaaggcgagattacgatgacatgagtccaaggcg
ggggccacttcctccacccctggtcgtggggtagagggt
3' EST: CX967043 [+]gacttgttttaactctggaaaaaaaactgctccactgtttactatataacaataaaaaaaaaagtcattatattaataaaaactcaagcacagctggtca
attaatatactacacaggcaactaatttcactaatttcaattacaacatagcaatgtaaaagtgataaaaacagtcaaggtcaagtgtacaatctgtaca
gtgttacaataaactcaaatccaacttttaaacagacatttcaaaatcaaacaatgaaaaaaactttcaattaggacatttgggaaaacttataaaaatc
aacacggggtgcagaaataaaaccaaacaaaaaagggctaaacaataaagctgaaattttaatttgctactgaaaatctgttctcacttaaggtttgcat
gggggaagattaagcagtctcatttactatgagaaattgactttagctcaacacaagtcagaaagaccttgataaatgcagatatattaactcagctcta
ctatttctgtacaattctcttggagcttgagtattttccgcgtaattgtgaattcttatcgatgctggtaactattaacaaaagcatccagagatccaga
tcttgatttgtactccattagcaaccaaagaggaagaaaagcactccaggatttcattgaagctgttctaaacaaaatattttggaatccacttatgcat
tagaacccttgagcatgtgcttcgcaatgtttagaagtcaaaacccataagcataatcttcagaaaactgcttcacactgttctgaagcaaaaattgtgc
attctgaatctgatcttgagtgccagtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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