XB-CLONE-3562185
Clone Name: IMAGE:7710774
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Gene:
clic5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967108 [+]
ttgttaagtacacccaacgggtatattctggtacaaacctctcacatccctgtgctttgcagggccatacttactaacaatctggagcagggataggaca
cctttgggctgttacagaacccccagcaggttccaaacttaccccaaggctacccgaggccctatggcagaaggttgaatattgacaggctcttagccta
tagcagtgttggccaaaaatgattttaattgaaatccaaggtgaatctctgcctggaaacgtacagacattttgggccaaaatacactggttctattgat
ttgaggacaccaagcacgcgatgagcctacacaaagtgtatcatggcagatgtgagcagttcctggaactccttctccctatatttcccagctgacaccc
gaagaccttgttgcttttgttgtcttcactccccggccctctctgcacagtagcagcttagtgcttgccggaattatccgctgccggggagtcaccctgg
tggccgtagctgcttcatgtgaccataaccagacaatggtgcagtccctcagctgctcaggggcaatagggcaataaacatacacctgaggctctggtat
aggcagatttcaccctgaaaatgtgcattggatttcaggccaacacctgccggtgccacgagcctaaacgggtgccacggaagttccccggctccttccc
ttctctatttaccattctttcagcgtctctttatcatctcagcacttaatatctctctctttatttccaacctttcaaaacatttccatccgtcctttct
gattctttccatcttcagtttcccatgtacgtggggtaaatccaaaccagtctctgcccccccagagtatattaccccccacta
3' EST: CX967107 [+]gactccataacttggctcccgggactcacccacctttcctgaccttcaatggggaagtaaagacggacgtgaacaagatagaagagttcctggaggaatc
attggcacccccaaggtaccccaaactggctgcaaagcatcgcgagtccaacactgccgggattgatatcttctccaaattctcagcctacattaaaaac
acaaagcagcaggacaatgccggcctagagaagggtctgacaaaagccctgaagaaactcgacacatacctgaactcgccgctgcctgaagagatcgatg
ccaacagccgagaggaggagaaagtatcaaagcggaagtttctagacggggatgagtttaccctggctgactgcaacctcctgcccaagctccatgttgt
caagattgtcgcaaagaaataccgcaactatgaaatcccagcggagatgagtggcatctggcggtacctgaagaacgcttacgccagagacgagtttacc
aacacgtgcgccgcggataaagagacagagctagcctacgccgacgtggcgaaaaggctctccaagtaattcagctctggaagcgaaattccccggcgct
aaagtgttctcacactcacagactgatctccttgttgccttatgatccatctacacaccaaatgggttgaacaattctgcttcgggataggccggctgtg
tgtgtgactttgaaatacgtcgccgccgactgcattggaaaaccaagccgtgatttcttactcttgagaggtggcgaactatgttattttttnaagggaa
aagttgatcagaatgagaaaaaaaccccgatatctctgacacccatcaaatcttaaggcaacacgcgctatctataaagtatttttgtattggaagctga
ttcctttatcagtgatttatgaaatgtat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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