XB-CLONE-3562113
Clone Name: IMAGE:7710924
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Gene:
eps8l3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967157 [+]
agaaatattactgaaagacccagaaaccaaggacagtctagagcaattacctctgcaaaatgttgataactgcagttctgtgttggggggctctacatac
aattcccttctcaccattaaagttcagactcaacagaaaacaagcatcttcttcttccaatgtgatgaacagccggctgatgttcttcatgcaaacctag
aaaaggcattaaagcaattcagattaaacaaagaaagcaaggatacctacaggtataacttggagaactctcaccctcaaaataattttttagaaaggag
tcaatcaccacaatccagtggaggctgggacagtcccagaatggaaaggcaaccaaaatactccccccgtgaatcccctattcagcagaggagatttgag
ctctctgaaccagtccatcctcctccaagcaccaagcctcctccatccttccaagcagcccctgtcaattcaaagtcagagacagaacgtgacattgaaa
ttttgaatcatcttcttggagacattgagttatttgttggaaaactgaatattgataagaagaaaaagaaagcaaagcttcctgaatcagaatttgtaga
ttgtctgcagaaagctaaatatgcctttattctaatggggaatgtccacgaacaaatgaaacaaccaagtgctccagatctcatccatttgatgatggaa
accctggccaagattgtagacaggtgtccacaaaaagacttgccacagtctgtgttatctccactgcttacacagaaggcactgatgattatgagttctt
gtgtgacagacaaagaaagaaaattctg
3' EST: CX967156 [+]ttaagtccaaggatcaatcgaaaaaaaactgtgacacaaagatacagcttcactccacatatttcgttttggcggtcctcacagatcgagtgtgaaaatg
tattgcaacttctcaacaatcccatagtggtttcatgtctcaagagatcctctcactgtgctcagttgcaaatatacacgacccccttcctcgggacaca
cagttttcaagtcttcacgagttagctccaaaagctgatctccttgaagaactccaagacatctcacagtgatattggagaagcctttctgttgaagcca
cgctttcacctcctctggacgagaacttggatttagtaccactgaacttgcaggagcctgaccatcatctgtgttctccaggatgttgcttggaataaat
ccctgctggccctgagaattctgcaccatccaccactgccggctctggtccaaaaccttcacaatgtctcctttccttactgacagttcgcgattatttc
tcgcttcaaagtcatgaaggacattcatgaggagtgggtgaaattgcctgtttcgtagtggtgtttcaggactacgtgacatctgggatgaaacatcact
aaaggggatatcagggatgatccacccatcagaaaaagttggaacatattgaggtatattctttccatttggccattcagctctggtcttcatccaggca
tctcccaggctttcccagaattttctttctttgtctgtcacacaagaactcataatcatcagtgccttctgtgtaagcagtggagataacacagactgtg
gcaagtctttttgtggacacctgtctacaatcttggccagggtttccatcatcaaatggatgagatctggagca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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