XB-CLONE-3562056
Clone Name: IMAGE:7710738
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Gene:
des.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967195 [+]
gagagttcataatactgagcggctcctatatgagctttggggacaaggggtccttagacagaagggacaagtgataggtgcagaaggtaatgtggtgaag
gtacaacatgagatgccatttgtccagcagggttgcactcaggctgtgccatgtgggtgttcttttcttcctgttgcgtgcccacggccagggagacaag
atggcggcttacaggatctcctggtgctgctgggaagcctcacttacaacctcaccatcccgggtctcaatggttttgatcatgactgttttctttgtgt
ggacttctgaagctctctgctctgggctggtttctcggaaactgagtgcagagtatgtttggatgggaagagtgatcctgctctcctcgccctcgagcag
tttacggtatgtggcaatttccacatccagcgccatcttgacgttcaggagatcctgatactcgcggagatgccttgccatttcatccttcatattacgg
atctcttcctccaaacgtccaatggagtcctggtacccagcggcttccccagaaaacctttcctcgaggtccctcatctggcgcatcaaggagtcgtttg
ttcccttaagtgcatcaatctcacaggtgtaggactgaatttggtggcggtattccatcatctcttgcttggcctggcgcagggaatcgttgttcttctg
agctgcctggttgaggtcactgaccttggacttgtaccactcctcggcctcagagacattcttggcagcgatgttctcatactgagcccggatatctctg
agtgctgccgtcaggtc
3' EST: CX967194 [+]gagaggctcaaaatccatcatgaaaaactacaagaaacagcttggcttttgcttttgctttttttttgcagaattaaagggcagaatcatataaataagt
tctctctttcgctctctttttttattttccaaaaacatcgcattatagtgaaatgtagcaaaaatcccaagtgtgggaggaacccgaggctgcggcacaa
ctaccaactgaacagacggttccccggccgggaaataacggtgaaaataaaaatcccagtacgtgcgtgggttctagggttctagtacagtgagaggaaa
ccatacggaaagcgctggcagtaaataaagagggttaattggatcatttctggtgccagaacctactttcatgcagcagactaaagtactgaatgctctt
atgaatgtggctaccagaatcccctgcggttccgcggggggcggagttcagaagcctttgtacatttggcaatgagtcccacctacatttgctgggtcga
gttcccgttccaggtgttgttctcgtcctcgtcgtcctgagttcgcagtctgtagattttgccttcttttttgaactcgtccgtgcgcttttctagaact
cttttcctaacaacatctctctttggttcggaactgctgctcgctgttgggtttgtgctttcgtgccgtggatgtgccggtcgctctgcaggctgggaag
gggaggggctaccaaacaggaagttgcttagaaaccgccatactgctaggagagggaagagaactgtaccgaggaaaccccagactccgccttcagagct
ctgtatgacagcgttggacggccttcctgctggcaagaggactattgaagcactgggtgcgagttccaacgancacagggatttcccataatccctcttg
gtgaatcccctcttggaaacat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: