XB-CLONE-3562037
Clone Name: IMAGE:7710740
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Gene:
dlg5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967209 [+]
catcgattcaattcggccgagggtttaatctctttttgaacccctattcaagtataacagtggaaggaatgcagtgctgacagttcccaaagttgcaaag
tgaaagcatatacagtatattacagggctcaagttttaacactcgtgaaaagcagtactttgcttcttagtgtctagtacctttaattagcacgtcagag
ccctcctgggataatgtcctggtccctagagaagaggcactcactgtgctcttgcattttatacatttctgtttaaaagtggccttttaaaattctcttt
aagatgtaagtttgttgccatcaggctttttttgttttctgatattcttgcagttttcagttttcttattcatctgtttgtccaaagagatggcagttgt
tcttgtgaaatctgttcacttttagttctggcatctaagcactttagtgatgtgcagaagtcttggaagtctgtccattatcctggttgcatacttcaag
taccagttatttgctaactgactccgtggccattcttgggattgactttgtatttatttaatggaatctaatgatttggtgatggataagtggtttacca
ataaacaattgttactgagtatatagtgttaaattcatcttaaaatcattaacttccagcaaaaacaggcacagttgaggatttctgtagataactgtga
ttacgtattaagtcataaagtgaaccgtgctctctgaactttacagggatcctgctggaatccatagagctttactttgttcttgatccacaatggagca
aatctgattgcaaatgctaaacaggcaccaccttgggatacagctgtaaaatacttgctgtattcctgttcaatnctgtgtgcagtttcaactgttcttt
ggaatgttttgtgt
3' EST: CX967208 [+]tttgccagcagtcaatcccaaagctcagttgcaagcctccccagaatatctgtcagtactgtgcccattggggaacgcaggaaggacaggccactagtgg
aggagccacggcatgttaaagttcaaaaaggttcagagcctctagggatttcgattggtggtggggagagtgggggaatatttgtctccaaggtaacaac
aggaagcattgctcagcaagctggattggagtatggtgatcagctgctcgagtttaatggcataaacttaagaaatgcgacagaacagcaagcccggcta
atcattggacaacagtgtgacaccatcactatcctggctcagtacaacccaaacatgtgccagcacatccattctcgttctagctcgagactggacacaa
gcaccactcattccacaccacacagcagtggagcagcaactcctgacaaccattccatcattgacactgtgagtgagcaggatgaagggaccatgactcc
accttccaaggaatctacacctagcactagcccatgcagctcactcagaacaaatgccgaaggaattaagagacattcagaacttcgtgtagtcaccctg
tctaaatctccagagaaactgggaatacaaatctgtggggggaacttgcatggaatatttgtcttagacatagaggaagaaaatgtggttggaggtttgc
ttaaaggagatatggttcttgagtatgggtctgtggatatgaggaaccgaacagcagaggaagcctaccttgagatgcttaaaccagca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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