XB-CLONE-3562025
Clone Name: IMAGE:7710932
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Gene:
eif3d
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967217 [+]
tcctcctcttcacttcctcttcttcctcctcatcatcatcatcatcttcctcatccgagctgaaagtgccatcaggtaggctatagatccgaataacttg
cttgtttggatctttcagtatcaggtattttccctcatccagcttcatgcagatgtccaccacacagcgcagaattccccatgcgttttccatgctcaga
ttaatctgattggcaaactcattgggcttaaactgttgagtgcccaggaccacatgacgagtggaatccttcacgtggcagcgggatacatagcctagtt
ttaggtactctgagccagccagcaaagcacagcaggtccaacgggccagtttgtagctgttattcttaagttcggtggcaatcactgcccctctctggga
gtccagcttctgtcgccagtccactccattacaatacctggaatcccattcattcagagtcttgatattgacaaatgacacctccccattggccccagtc
atgaaaccatcatgctcacaacgcaccaccaagtcaatatcatccccgagcttccaacgtcggtacctatatgctacagatgctacttcattcttgtcca
catcatcctctatgaagggatttgggttgggaaatgtgtgcttctctttgcccatgcgaaggcattgctgggagaaattgtggttgatgtaagtggcctc
catggccagattccgtggggagtt
3' EST: CX967216 [+]tgggatggggcccatgcgctgtacccgatcagtttaaagatatgccataccagcccttcagcaaaggagaccgactgggcaaggttgcagactggactgg
ggccacatatcaggacaagagatataccaataaatactcttcccagtttggaggtggcagccagtatgcatatttccatgatgaggatgaaacaagcttt
cagctcgtggataccacaaagatgcagaagactgcataccaaaggaacaggctgcgatttgcacagaggaatctccgtcgggacaaagacaggcggaata
tgctgcaatttaacatgcagacactgcctaagagcgccaagcagaaagagagggatcgcctaagacttcagaagaaattccagaaacagtttggtgtacg
tcagaagtgggatcagaaatctcaggcccagttgaaacctcgtgattcctctgtagaggtgcggagtgactgggaggtaaaggaggagatggacttccca
cggctgatgaaaatgcgatacatggaggtgtctgatccattagacatagagtgctgtggggccttggaattctatgacaaagcattcgatcgcattacca
ccagaaatgagagacctttgaagagtattaaaagaatatttcacacagtgactaccactgatgatcctgttattcggaagttggctaanactcagggcaa
tgtctttgctactgatgctatacttgccacgcttatgtgttgcacacgttcagtcaattcttgggacattgtggtccaaagggtggggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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