XB-CLONE-3562019
Clone Name: IMAGE:7710646
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Gene:
ermp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967221 [+]
agtcatcaaaatttattaaaaacagatatggtgaaaggtttcactttacattttggttagaatgacctgtaggtatttagggtatagaaaacacaagtta
gggcaaaaaaaaaaaaaagcaaagaacccatttccaccttttactataaataattaaagcagctccacaggaaatattaaaaggaattcaatgtgtttgc
tatgtaatcatataaccagcaatacagtagcaaaaaagaacagcaggttcctaatatgattcaactagatcaaaagccattctctacatagcaaatcagc
tcaaatacacaatgcaacatagacaaatacacattcaggaacctgtatatttgctaacagacaatagctgccttctttacagtaatggatgagataggca
gaacaacatgaaggcatgtagtagtgctcagtatttgcaaaactcatgaataagtctagaatgtgcaggacaatgaatgtatcgcccaatatatgtacaa
cctgtaataactattgtagcacaactggtgcacacaagagaatattatttttttcagcacagtaaaaaaactgcttatcattaacttttttatgttcatt
gtttcattgggcaggagtgagcgtgaaggaaaacatgtttttttttttttttagaaacacatcagttaagagtttctccagcagaatactgcaatgaaat
ccatttttcaaaaacacagatttttttttatatttaattttgaaatttcacatggggctagccatattcttcatttc
3' EST: CX967220 [+]cagaaattccgcctgacgttgtgctaggtttcctgattatcgcatgtaccataatgctgatatcgtactttattagctttatctatctagtaaaaaacac
caagaacattattgtacctttggctgttttatcagttcttacattcctgcttgtctgcagtgggatgttcttcccatacagctccagtactgacagccct
aaaccaaagaggatcttcctgcagcatacaacccgaacatttcacagtttgacaggagaagtggtaaaaaaagattctggcatatggattaatggctttg
attacactggaatatctcatgtcactcctcattttcctgaattaaatgatactgtgcgtgccccatgtgatgatgccccactctgtggctttccttggta
ttttcctgttcacaatttgatcagaaaaaactggtacctcccagccccaccagctcctatctacgaacacacagaatttaaacttgtgtccagggaggag
atgtcttggggtactacaagactgcattttgaggcgaaaggaccaagtcacatgactgtatatgtacgcccacatgctgactctgtattgtccagttggt
ctcttggtgatggaattcctatagccagtggagaaaaggactattttatattttactctcacggactatatgccccagcatggaagttttggatagaagt
taagaactcagctgacgaaagtaagggaatagtgacagttgcagtcggttcacattacttcttcggagacagtcgcaattctcctcatctgaaaacagtg
gagttgaaatttccagactggagtttcccatcaagttgggtgtgcacctatgacctctatgtctactgatatgttggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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