XB-CLONE-3561967
Clone Name: IMAGE:7710746
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Gene:
tap1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967257 [+]
atttccataagatcaagtacatattaaccacgagtgctttaataaataaatcccttgcaccttatctcttactaaacaatagcagtctttggagtctttt
ggagttatggaaaaaataatgtattgttagacaagaacatgctggtttgctgcttttattcacactttatgcttagaacacaatagtatatgggaccatg
tttttagttctgcaatcaaaaaccaaccacaggctacttaaacaatctaaataaaaaaaattcaagagatatcaagtctttaggatgaacaaactgataa
ccatgaatacattcttagactactgctgtctgtcattccctttgaaaactgctgaattgcttttgccatagtttccagtagctgcccttctgtgccatta
gctgttcatgtgtcccctcctctgtgatacaccctccttcaagtactaggatatgatcagctctctgcacagtgttcattcggtgggagataagtagaac
cgtcctctctgcaagttgatcatagatggcattttgcaccttcagctcagactctacatctagtgagctagtggcatcatccagtatgaggaccttaggt
ttcctcagtaaagctcttgctaatgctacccgttgcttttgccccaccgagagcaggcctccttttggcccaacacctgtttggtagctatttgccattt
ctgagatgaaatcatgggcgtctgcagtctttgctgcctcctgaacacttctgaaagaaacgtttcccataccgtatgcaatattatcttgcagagagca
ggccgagagagtaggctcctgactaaccagtgatacctttttgcgatagtactgattgtcgta
3' EST: CX967256 [+]gaggtttcaggctccttactttatctataaatagagaagcggcagtgtaagattgcgggcagggagggtgagcggaagagctcagcccgaacccgcccga
cccgagggttaacttgtgggttccgcgggtatcaggccaacccgcacatcactactggtgatgcaggcagctactcttaagtaagttactttaggctaat
gttatttaatactacgtatttggagcactgtggtagtggtagacgcgatggtgctgggttctaaatttttaagtggttttatgaaatttctttctgttaa
aaggctttttttaaccaataaaattgtgcctgattattatacctaggcatggctctatataaaagaagtattttacagtttttatgtagagttatatttg
taaatgtaatcgctataatcagtatttgttgtttttgttcaaagagatgtatattcaaatatgggggcatatcggggtagcctaatccactacatttaat
tctccattgcaccttggcataactcagttgttctctttgcagattcaagctcaccatgaatcccttctggactctgagttgtctttttgcaattgatatt
gccaccttgcccttaataggatcatttctctctcacatcttaccttcagatctccctctaatattaccttggctggttggcttgtcacggttgctgtttt
tgatcttgagtttacgtgtactcagtagaagcttcaggctcccattccacagagatcatctaaacctgtactgcactgtactaagtctgcttgttcctat
ttatattagtctcacctctttagtgaacccacatagaagtgccaatctgctttacagctggactcactgggtcatcttgttctggtgctacctgctcaca
ctgatttcagcagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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