XB-CLONE-3561919
Clone Name: IMAGE:7711133
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Gene:
ace
,
arcn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967288 [+]
cggcagcagtctgcaccaaggccggtaaggccattgtatcaaggcagtttgttgagatgacccgcacacgtattgaaggacttttggccgcttttccaaa
gcttatgaacacaggaaagcagcacacatttgttgagacagaaagtgttcggtatgtttaccaacccatggagaagctctacatggttcttatcactacc
aagaacagcaacatcctggaggaccttgaaacactcagattgttctccagggtgattcctgagtactgcagagctctggaggagacagagatttcagacc
actgctttgatctgatctttgcatttgatgagattgtggcccttggatacagagagaatgtgaacctggcccaaataagaactttcacagagatggactc
gcatgaggaaaaggtgttccgtgcagtcagagagacacaggaaagagaagccaaggcagagatgcgacgcaaagccaaggagcttcagcaggctagaagg
gatgcagagcggcagggcaagaaggctcctggatttggagggtttggaagttcgggtggttcctccatgtctaacaatgcagtttcgctgattacagaga
ccattatagaaccagagaaaacaaagagtgcccctgttccatcacggccatctggttctggcaaagccctgaaacttggagctaaagggaaggaggtgga
taactttgtggacaaattaaaatctgaaggagaaactattgtcaccccttcatctggcaagaggcagtcagaagtggccaaggtgcttgctcctcccgtg
aacatggagagtgttcacttgagggttgaaagaaaaaattt
3' EST: CX967287 [+]aaattcttggagatgttctccggtcaggatcctccaagtcttggcaggaggttctgaaggacatgacaggtagtgaaaagatggatgttgggcccttgct
tgaatacttcacccctgtcacacagtggctgattgagcaaaacaccaaaaataacgagattctcggctggccggatttttcatggacgcctccaataccc
gatggatatccaggagatattgaaaaaattgcaaatgagaaagaagctgatactttcctgtccgattataccaagtcagcagaggtggtgtggaacgatt
atacggaggcatcatgggcctataacacaaacatcactgaggccaacaagcaagttatgctggacaaaaacctggccatgtcgggacacaccttacaata
tgggctgcaggcaagaaactacgattacagtgatttccaggaccctgagacacagcgcattctgaggaaactcagtgagatcgataaagctgctctttca
gaggaggaacagaaagagtacaatcaaatcacatccgacatggagactatttatagtgtggccaaagtttgcaaggatggtaacacaaactgccatcctc
ttgatccagacctaacagagatcttggccaagtctcgggactatgatgagcttctgtttgcttggaaagggtggagagatgcttcaggcaaacaaatccg
tgaaaaatacaagagatatgtacaattggccaacaaagctgcacagctcaatggttacaatgacaatggagcatactggagatcttggtatgagaccccc
acacttgaatcagatgttgagaaactttatgacgagttgcagcctctctaccctcatttgcatgcatatgtgcgcagggctctttacaagaagtatggag
a
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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