XB-CLONE-3561908
Clone Name: IMAGE:7711325
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Gene:
fip1l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967295 [+]
aatatatggaccaacaggcaggaaaaggtaaaggggtagatttggatgccccaggaagtattaatggcgtgccgctgctggaagtagacttggactcttt
tgaggacaaaccttggagaaaaccaggtgctgatctgtcagactattttaattatggttttaatgaagatacatggaaagcttactgtgaaaaacaaaag
aggctaaggatgggcttggatgcccttccagtttcttctacaacaaataaaattacggtacagcagggaagaacgggcaatgcagaaaaggatacagtct
tgcagagtgtgaaaccagagtttacttctcccacttcattattcaaacctggtgttcctcctccaagtcgaaaaacctcaagcgcaattgatgtgattgg
tcagacaaccactatcactcgtgtagagggacggcggcgaggtgcaaatgaaagcaatatccaggtagtttcagaacagtctccttctgaagtggataat
aattttatcaagccacccccaccatttttccctcctggagcaccccccacacacctaccaccacctccactttttcctccaccaccaaatgtcagtactg
ctccacctctcatcccacctcctggatcaatatcgttctcgatgcaaaaaccatctcgatacggaattcctatcactgtgccacctccaggtttccctcc
tcctcctggaggcccgccaccatctcttatcccaacaatagaaagtggtcactcctctggctacgatgggcggtcagctcctgcattttcttatggcagt
gtgtcttttcctcacatgcctggttctgct
3' EST: CX967294 [+]gcaaagtgaaaaaagtcaaactattgaaccactacaagtatttttctttaactggggaaaaatatttatactttcaacactcatgtaatggcttccggac
ctcagtgcaagtttgttaaaaacggaagtttttgcacaaaacttgggtcatgcgtgcctaatcggcattggtggattcaacactttctgattcttggaca
ggttcggccccagcatccttcccttctttgcttcttttggactttttgtgtttgtgccttctgtgactatcgccctcttcgctatcatggcggcgcctgc
tgcttcgggaagatttgtgacgagacgactcctctttttctcgatgcctcctttccctgtgcctttcctctttttccctgctgattctctctctgtgacg
ctcatagctcctctccgtacgctcactatactccttgtatctgtatcgctcttcctcactattatatgcgcctgcgcttggactgtgatctcgctctcgt
tctctgtcccgttccctctcacggactcgctcacggtctcggtctctgtctcggtctctgtcacggtctcgctccctttcgcggtctctatccttctctc
ttcttggatagtagtcccatggtttggcggtatctaccagactggaccacggaccagcagaaccaggcatgtgaggaaaagacacactgccataaggaaa
tgcaggagctgaccgcccatcgtagccagaggagtgaccactttctattgttgggataagagatggtggcgggcctccaggaggaggagggaaacctgga
ggtggcacagtgataggaattccgtatcgagatggtttttgcatcgagaacgatattgat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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