XB-CLONE-3561895
Clone Name: IMAGE:7711183
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Gene:
hsp90ab1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967305 [+]
acaacaacaaaaaataaaataaagttccgtattgaggctctcgatagagagatgtgtaggatgggggtggggtctcagcattcaagacatcctgggggcg
ggggcacgagattccagtcagagagatgggggctaccactcagcagatttgggggtgctacagatggaggtagctttgggcagaagtacctcaccccccc
agactggcctggcgtttctatttgcaaacaccagcacattcaatcgcccccccccatgcaagtgaatgggagaaggcagcaggggggtctctaaagcttc
ccagtaagtaccccccgtgcctcattaacctatcaggcacagagaggggcacgcccattacaatagtggcagagggtaggattagaggggggcctgtaag
gtgcattgagagagtcgaacagacccccccagcagctggctaacagcgggccactaactcagggctaccacaagccaagctagctgccatactgctacaa
taacttacataactaactgctcagactggaacacaacaaggggggtgcagaggaaagggttaaagtcagcagccatcagtcttgaagctcccccaaatat
attctataaggagggaagtccatccccagtatcatgtgatatttagagtccaagggagaagtcgcccccctaccgagttacagacagtgcacatacgcgg
gtgggagggggcaggacgagcgggcagcaaaccgattagtcgacttcctccatgcgagaagcatcttcgtcgccctctaggggtgggatctcgtctgcca
ccgagggggaagtttcctccaccgtgcatcgtcatcatcaata
3' EST: CX967304 [+]tccgcgctctgctcttcattccccgccgcgccccctttgatctgtttgagaacaagaagaagaagaacaacatcaagctgtacgtgcgccgcgtcttcat
catggatagctgcgacgagctgatccccgagtatctgaacttcattcgcggggtggtggattctgaggatctgcccttgaacatttcccgtgagatgctg
cagcagagcaagatcctgaaggtgatccgcaagaacattgtcaagaagtgcctggagctcttcaccgagctggccgaggacaaggagaactacaagaagt
tttacgaggctttctccaagaacctgaagctcggcatccacgaggattctaccaaccgcaagaagctgtctgagctcctcaggtaccacacttcccagac
aggcgatgagatggcctccctaacagagtacgtgtcccgtatgaaggagaaccagaaaagcatctactacataactggtgagagcaaggaccaggtggcc
aactccgccttcgtggagcgggtgaggaagcgtggcttcgaggtggtctacatgacggagcccatcgacgagtactgcgtgcagcagctgaaggagttcg
acgggaagacgctggtgtctgtgaccaaggagggcctggagctgccagaggacgaggaagagaagaagatgatggaagagaacaagaccaaatttgagag
tttgtgcaaactgatgaaggaaatcctggacaagaaagtagagaaggtcaccgtgtccaacagactcgtctcctctccctgctgtatcgtcaccagcacc
tacggctggaccgccaacatggagcgcatcatgaaagcgcaagctctgcgggacaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: