XB-CLONE-3561772
Clone Name: IMAGE:7711147
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Gene:
hnrnpdl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967386 [+]
atcaaaattgaatgcaccgatcacttcacaagcacaaacaaggaacttaaaaatattgcgaacataaaaccctctttcatacagatggcaaaaaaatgcc
attgaaataaaaaaaaaaaaaaatcccagcttctatttacagcaagacacttagtgcctcagtcatagtcctctcaaaagagtaacatgatggcggctcc
cggcattgtcgcaggatgttaagtctctttatccattgccacctgaaacaaagaaaagagaatccttaattttcaatcagaagtcatccacttcacgagc
ccacatttctacaaagtaaaaatcatatgcacattaaatgctatgtacagtgtgtaaaacctaactttagtttgacatttatcgctgatatatccaacat
ctctgaagctgtaacctcagaagtggtcaagcttgtaaactgtataaaggattcattaggtctgtgcataggattttttttctttttaaatttacaagac
atatcacccctcattttaacaagttcttgtttgtctgtagattttttagctataaagaaaagaaataagcattaaatcctataaacgcacaatttaggaa
tacaattaaattaaaacgaagagcaaaaaagattaagctgtaaaggcatctaactttttaaactcctgtaaaaaacaatttatcaaaatgttaacaaaaa
aggactccaaaagattaaaaaagccaaagcctgtagttgtttttcccagtttaatttttacgctggtacatggaagttgg
3' EST: CX967385 [+]ggcccggacttcaacgaaggctcaaaaatcaatgccagcaaaaaccagcaggatgaaggcaaaatgttcatcgggggtctgagttgggatacaagtaaga
aagaccttacagagtatctgtctcggtttggggaggtggtagattgcacaatcaaaactgatccagtgactgggcgttcaagaggatttggctttgttct
tttcaaagatgctgtgagcgttgacaaagtgagtaatctgcctggctggaggtgtattgtctgtgcctaaaatcaagttaacttatcacttaatatttta
ttaaggtgcttgaaacaaaagagcataaactagatggcaagctcattgacccaaaaagagctaaggcactaaaaggcaaagagcctcccaagaaagtgtt
tgttggcggactcagtcctgaaacaacagaggagcagatcaagcagtattttggtggatttggagaaattgaaaatattgagctgccaatggacacaaaa
acaaatgaaaggagaggcttttgttttgtcacctatacaggcgaagaaccagtaaagaaacttttggaaagccggttccatcaaattggtacaggaaagt
gtgaaataaaagttgcacagcccaaagaagtctacaggcagcaacaacagaagcagcagaagggaggaagaggagcagccactggaaggggtggtgccag
aggaaggggcagaggtcaaggttggaatcaaggctacagcaactactatgatcagaactatggaagctatggaaacaatggttcttatgctgatcaaggg
tataataacagttatagtggttatgattactctggatataactatggaagctatggatataatcaaggatacacagactacagtgggcaacaaagcacat
atggcaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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