XB-CLONE-3561692
Clone Name: IMAGE:7711107
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Gene:
got1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967441 [+]
gccgaggcttcacagaatgtgtttattacattgaattaaccgctttagcatgttcaatttaagacaatagttttggttttccattgtgcactctagcaat
gatgactagattttggagaccttagttctaagatctgtattgcaccatctgagaatgggctcagacatgtagagttaatgttctagccaatgttctatat
acagttcctggatatttctacgtatcattcctgcagaaggggctctttgcatagttaactattgcaaccaaatagactttagttgtgataccatgcatat
ctagagcataacaaaattcattaaagcttgctgccaaggacaccttgatgcaggatttctatgacaacgaaaacataatttgctatggttgaaggttttt
gaaggttatatctaattttctataaattacagggttacaagatgatgcagaacaaatacgtagaattaagatgtcaatgcagtcattaggaaacatttaa
aaaaacaaaacaacttgagtgcaaaatagtgtaaaagcccaaaaaatcacaatatatagcatggtgtcccacggaacaaacatgctagcacttgtaacac
atctcccagcagcttcattggttcctgttaaagccatagataatgaggtgatcagatatctttgtgactgtgagcttggggacacgcactacaacaacac
ataattacactgctatttcatctactgaaagcttgnggtttctttactggatcttggttgctgcttcataaatagactgggctacatagtcgaggttctt
tgttgtcagcccacacatatttatacgtccactggccatcaagtagatgtgcttctctttaataagatac
3' EST: CX967440 [+]atctcgagggattcctgcgggatttagagaatgcccccgagttctctatctttgtgttgcacgcatgtgcgcacaatcccactggaacagaccccacccc
tgatgagtggaaacagatagctgatgtcatgaagcggaggtctctctttcctttcttcgactcagcctatcaaggatttgcctctggcagcctagacaag
gatgcatgggccgtgcgtttctttgtgtctcagggttttgagttgttctgtgctcagtctttttccaagaactttggcttgtacaatgagagagtaggaa
acctaactgttgtagggaaagactgtgataacgttgcacgtgtcctgtctcagatggagaaaattgtccgtacaacctggtcaaatcctccatcgcaagg
agctcgtattgttgccaccactctcaacactcctgaactatttgatgaatggagagataatgtaaagactatggcagagagagtcctgctgatgagagca
gaactgaaatcccgactggaggctcttaaaactcctgggacatggaaccatattgtcaaccagataggaatgttcagctacacaggacttaaccctaagc
aagtggagtatcttattaaagagaagcacatctacttgatggccagtggacgtataaatatgtgtgggctgacaacaaagaacctcgactatgtagccca
gtctatttatgaagcagcaaccaagatctagtaaagaaaccccaagctttcagtagatgaaatagcagtgtaattatgtgttgttgtagtgcgtgtccca
agctcacagtcacaaagatatctgatcacctcattatctatggctttaacaggaaacaatgaagctgctgggagatgtgttacaagtgctagcatggttg
tt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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