XB-CLONE-3561599
Clone Name: IMAGE:7711307
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Gene:
kyat3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967505 [+]
tctgaaggtaccgtaacctgctccgagccctgtaagaaacgtaatgagcaatgtcttcaaagcacaaaaatgctaaacgaatccagggacttgatggcaa
tgtctgggttcaatttacagctctagcagcagaccccactgttgtaaatcttggtcaaggtttcccagatatatctcctccttcctatgtaaaagaaggc
ctagctcaagctgcagccattgacaagttgaatcagtacactcggggctttggtcatcccctgcttgtcgaagctttgtctctggtttatgggaaagtgt
gtggaaggaagataaaccctaacagtgaaatccttgtgacagtgggtgcttatggatccctctttagtgcagtccaaggacttatcgaagaaggagatga
ggtgattataattgagccattttatgacttctatgaccccatggttaaaatggctggagccacacctgtcagcatcccactgagatataaatccagaggc
aatggcgttatgtctagtgctgactggactttggatccagaagaactggctagtaaatttagtcccaggactaaggcaattatattaaatactccaaata
accctatgggcaaggtatatactaaagaagaactgcaagtaattgctgatctctgtataaaacatgacgcactgtgcatcagtgatgaggtttatgaatg
gcttctatacaatggaaataagcatgtaaagataggtactagtatccctttcctctacatataatggagaaaatagtttataatcaagattccatttaaa
atcaatgtattgggctcttgaaaaactgattggagcccatggatatatcatatgtg
3' EST: CX967504 [+]aattttgaaacgtgttaatttatttttaaaaacatatacatcattgttttgtacaataatattctaaattaaaaaatatatatattgttatccgttggac
aagatgggatgtctcatctttgtcagtgagcactggttttattggctagtggctagtaacagaagtacaataacatcacttttgctttagtaacagtccc
catctattgattttctgtaccatcaaacagagatgctggaagtgttttattgttaaaagaagcataatatgttatggcacatcccatactgaaaccattt
tgttaaggggattctcttcaacaaatgcttctcaatgtaatgctgggttaaagagctcttgctttcattttcacttattccagtttttcaagattacctc
tgcagcatccaatgtgctatcttgataaaacaaaaccgtatgtacttctcaaaaagctttgtagtttctggtccacaaaatgctgttattggaatagctg
ctagtttcttcattttaatcatccatttcacaaacttgtaatcgtaaggttcatcttccttcatatcagaaagatccacatctaaaactgaaatatcagc
aaccataaaatagcctccttctggaatgattggctttattccggcttcctggagcaagcgagccatccgatctctcttagcttccagctcccggggaagt
gagttgaaataacagtctggttcatccatccttttaaaatccttgaaaagagcatgagccagggcttcttgcaatggagttggacaggtgtatatacaat
tctgttggactgtctgcnaatgtcta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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