XB-CLONE-3561483
Clone Name: IMAGE:7711079
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Gene:
slc7a8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967585 [+]
atttccaggagccagacattggtctgattgcactggccttcttgcagggatccttcgcttacggtggctggaatttcctcaactatgtcacagaagaact
ggtgaacccctacaagaacttgccccgtgccatctttatctctattccacttgttacctttgtgtacgtatttgccaacattgcctatgtgactgccatg
tcaccccaggaattactggcatccaacgctgtggctgtgacatttggtgaaaagttgctaggtgttatggcctggatcatgccaatttctgtggctctct
ccacttttgggggagtcaatggatctctctttacttcatccaggttgttttttgcgggagccagagagggccatctcccaagtgtattggcaatgattca
catcaaacgttgcactcctattccagccctgctctttacttgcatctccactcttctgatgttagtgaccagtgatatgtacactcttatcaattacgtg
ggcttcatcaattacctgttttatggagtaacagtggcaggacagattgtcctacgatggaagaagccagacattccacgacctattaaggtgaacctga
ttttcccagtgatctacctcctattttgggccttcctactagttttcagcttatggtcagagcctgtggtctgtggaattggccttgcaatcatgctgac
tggggtgcctgtctatttcttgggagtgcattggcagaacaagcctcagtgctttaataattttgtggatgccatgacacgtgccggtcaaaagctatgt
gtggttgtgtatccccaaatggacaataaagatgaggatgtgagtga
3' EST: CX967584 [+]tggattggaggcaggactgagaataccctgaagtaacctgggtcagtcaggtaaggaggtggggtaaagtatttgattggtaaagagagacctgagacta
cactagggtgtactggcgtcacactttttcacttcctgttgctttcacaggaaatcttcagcaagagaagcatgtttgtcctgtacacgttacatgattg
tccgttgataacccagctccagcagcacagtctgtattggagatggtttaacaggcagtgtggagcagccaaatgatacacaatccatgatttgtgaaat
ataggatctgccaatgttcaaataacaaaaaaagttccatagattcttcagtcttttacatttcaaatgatttgtaaccctcaaggagcaacatgttgcc
aatattgggatgggtgggcaaaaggaaaatacaaatactgcaagccaagacctcaaatctgcacgttcacaaagtacacagacaatattccacaatgctg
agcagaactgctgttacttagctttcttccttccaagacagcacatactgctaattatggcatcgcaaggagtggttgacttacaaagtccaacagtaca
aagcacagacaaaactgcacatgtaactgacagggacaattatacacctacaacttcaaattatattacctgtcccaggnggcccccaaattagggcaga
tggtataattaggacgatggtgcaataaccagtactggaaagtaacacaaagaggcacacaggggtacccacacagacacacattgtcatatgcacataa
acacattcacgcaagttccttccatcactgcagatctgtacactgaggtcttgacagcgggtatatgggggtcaagggagatggctgaatgctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: