XB-CLONE-3561462
Clone Name: IMAGE:7711129
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Gene:
tsc22d3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967599 [+]
tattgaggagccgccgtgccaaaaacatgagcacagaaatgtgtaacttcccaatggaggtggccgtgtatgagctgcacaatttctccatctcgttttt
ctcctccttactgggggctgatgtggtgtccgtaaaactggataacagtgcatcgggtgccagtgtcgttgccatcgacaacaagattgagcaagccatg
gatttggtaaagaaccatcttatgtacgctgtgcgtgaagaggttgaggtgctgaaggaacagattaaagagctggtggagaagaactcgcagcttgaga
aagagaatcatctactaaagaacctggcaagcccagagcaaatgaagaagttccagtctcggctccctcaagatgaaactccaatcaagacaaaactaga
tttccttcgcgcatctcagaaggctggctctgcagtctaagtggctctgtctttggagtgggcagagccactgaacttttcctgcgtctatatatgagaa
aaaaatgagatttttttcccccctctttaaagtgtcacctcggcttggagggtcgttgactagccaaagctggcatgtgccggggctagcgtgggcctcc
tcacaagtcttttcttcaagagaagagtgagagaagctagacatggactctttaagaagagtaccagccagagagagctggctcggcatgaatgttgcct
ttctttggcgccaggcttgggaagcaaagccaggaaacaagcaggacatgctaaagatgaccagagactgcttctgcaagagttctctgggcatggaata
caaagttgatgaggagatctccctctct
3' EST: CX967598 [+]tgcagctttccactgaattatgtacaatccagaaatctcagacagtaatgttaaacagcagaaaacattattcgtccaacaagcccttttatggccattg
ctgggagctgtagtcctgaagtttttaggaatattgcaaaaaaagtccacagtctattaattttcccggattttaaagtgtcttgtgtgagtctttcagg
cggcttttcccaccattgtgctctggcatagagtcagacaggtacatttatattctacacatacataaaaagacaaagtaaaaaaaaaaaaaaaagtctg
tgtagcatattcccaagttctcaaagagaacattttggcaagtgctaaaattacatactagctatgtaaacttaaactaacaagcccagtagtgggtggg
gtctaaagagactgccaccgatcactagtgtgggcaactgtgagccaacgtataacaatctccattataaagcactaaagtgccctatgtattatacttt
gtactgaactgttactagggtaactgttccttatcctcattccaggaggttaacgctctctgcttcccactatggtacagtttataaaaacacacagaga
gggagatctcctcatcaactttgtattccatgcccagagaactcttgcagaagcagtctctggtcatctttagcatgtcctgcttgtttcctggctttgc
ttcccaagcctggcgccaaagaaaggcaacattcatgccgagccagctctctctggctggtactcttcttanagagtccatgtctagcttctctcactct
tctcttga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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