XB-CLONE-3561405
Clone Name: IMAGE:7711326
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Gene:
fip1l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967636 [+]
tccatcgattcaattcggccgagggaatatatggaccaacaggaggaaaaggtaaaggggtagatttggatgccccaggaagtattaatggcgtgccgct
gctggaagtagacttggactcttttgaggacaaaccttggagaaaaccaggtgctgatctgtcagactattttaattatggttttaatgaagatacatgg
aaagcttactgtgaaaaacaaaagaggctaaggatgggcttggatgcccttccagtttcttctacaacaaataaaattacggtacagcagggaagaacgg
gcaatgcagaaaaggatacagtcttgcagagtgtgaaaccagagtttacttctcccacttcattattcaaacctggtgttcctcctccaagtcgaaaaac
ctcaagcgcaattgatgtgattggtcagacaaccactatcactcgtgtagagggacggcggcgaggtgcaaatgaaagcaatatccaggtagtttcagaa
cagtctccttctgaagtggataataattttatcaagccacccccaccatttttccctcctggagcaccccccacacacctaccaccacctccactttttc
ctccaccaccaaatgtcagtactgctccacctctcatcccacctcctggatcaatatcgttctcgatgcaaaaaccatctcgatacggaattcctatcac
tgtgccacctccaggtttccctcctcctcctggaggcccgccaccatctcttatcccacaatagaaagtggtcactcctctggctacgatgggcggtcag
ctcctgcatttccttatggcagtgtgtcttttcctcacatgcctggttctgct
3' EST: CX967635 [+]gcaaagtgaaaaaagtcaaactattgaaccactacgggtatttttctttaactggggaaaaatatttatagctttcaacactcatgtaatggcttccgga
cctcagtgcaagtttgttaaaaacggaagtttttgcacaaaacttgggtcatgcgtgcctaatcggcattggtggattcaacactttctgattcttggac
aggttcggccccagcatccttcccttctttgcttcttttggactttttgtgtttgtgccttctgtgactatcgccctcttcgctatcatggcggcgcctg
ctgcttcgggaagatttgtgacgagacgactcctctttttctcgatgcctcctttccctgtgcctttcctctttttccctgctgattctctctctgtgac
gctcatagctcctctccgtacgctcactatactccttgtatctgtatcgctcttcctcactattatatgcgcctgcgcttggactgtgatctcgctctcg
ttctctgtcccgttccctctcacggactcgctcacggtctcggtctctgtctcggtctctgtcacggtctcgctccctttcgcggtctctatccttctct
cttcttggatagtagtcccatggtttggcggtatctaccagactggaccacggaccagcagaaccaggcatgtgaggaaaagacacactgccataaggaa
atgcaggagctgaccgcccatcgtagccagaggagtgaccactttctattgttgggataagagatggtggcgggcctccaggaggaggagggaaacctgg
aggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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