XB-CLONE-3561378
Clone Name: IMAGE:7711042
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Gene:
XB5993342
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967654 [+]
tagatgttagattcagtgctgggagtttcacaaggaaacagaaatccagagagattcactaggaagtcaggaatccggtaaggaaggtccgccatgttgg
gttcggggtttaaggcagagagacttcgtgttaatcttagacttgccattaaccgactcaagctgctggagaagaaaaaaactgagatggcactgaaggc
cagaaaggagattgccgactacttgtcttgtcgaaaggatgagcgagcacggattcgagttgagcatattattagagaagattaccttgtagaggccatg
gagatcttggaattgtattgtgatttgttactggcacgttttggcttaattcagtcaatgagggaactggaccctggtttggctgaagctgtttcaacgc
tgatctgggctgcaccgcgcctccagtcagaagtctctgaattaaagatagtggccagtcagctgtgtcataaatacagcaaggagtacggtaatctgtg
tcgtacaaatcagattggaacagtcagtgacaagctaatgaataaactaggtactgctgcacctcctaaaatcctggtggagaaatatcttattgagatt
gcaaagaattacaaggtcccatatgagccagatgcagtaattatgggtgaggctcccacattttcggaggttgaccccagtattatcgggtttgttaatg
atgataagaagggaggtcctggcaatggaggagggtttggaggaggagacttcagaattaatcaagtttacccaaatccaaatgatccaaaatccttttc
tgatccttctgtagggatatatgaggctt
3' EST: CX967653 [+]ccatattatgtatttttattgccgcataacaaggggaacttattataatcacatatttataaagtgccaacatattatgcagcattgttcaataaatggg
tatatacattagacatacaagagaacatacaaaaacaaccaatacaagagttaaaaagggccctgcccaacagatcttgcagtcttaaagggaaggggta
tgagacaaattggaagaaaaacctacaaatgaatgcacaaagtctgacttcctttttttgagtgttgatatcatgctatgggtatccaaatgaccagtgc
aaaagaaagttcctggtttaaatatttacaatgagaatatctgctctataacaagagatcacaacagatcctttaggtcttctttttcagattatccagc
ctttttgacagatcctcaaaatcaatgtcttcatatggtgatgggtcagaaggtggggaaccgtgagccggactgctaggtacagatggcagtttaggta
gtggcaaattgttatactttcctggcacgttttctccttgaccagaattcattgcaggactagcccctgagtctccacctttgttgttcatcatgttaga
ggatggaggaatatctggaacatgcggaatcatttttccccttggtggtggttgtagaaaagcacaaggctcatatatccctacagaaggatcaggaaag
gattttggatcatttggatttgggtaaacttgattaattctgaagtctcctcctccaaaccctcctccattgccaggacctcccttcttatcatcattaa
caaacccgataatactggggtcaacctccgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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