XB-CLONE-3561346
Clone Name: IMAGE:7711284
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Gene:
mhc1-uaa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967675 [+]
taaatattngggttgttataacattcaatattcagcattctggtgtgatcatattgcttaaatataagggtgtaggtttatgttgtttaagttttaaatg
tttttgatttttgaattgtaaaaatgaatgtaaaacatgtgcaacttatccatgtagcacagtgtatggtagaattatttgattacatttgcagggattg
tataaagctaagaacaaactggttcgtcagttgtataatttccttttctacccagaataccaaactggcatctgagtttcatatttcttctcataaactc
ccacggaatgtcacctactgtatcttgtattggcagtgttgtcatatttgccattattattattattattattattattttacaagtgatttcatgtaac
cttctgctcaattggctcaattatctgtccaaccagtgcatacaggaattatgcaagcaccttaccttatagcattcatggcctcccagtacttggcctc
ctctctcctgcatgccctcagctttcagtgctcatgttttttctacaccctaaagtctaacaagccctcttcaggagtatgtcatacacagctctcagca
ccatttttcaaatttagaaattcctgaacaaaccccttccttactgcttctgtttccaggcatgacaagttccaggattgtagaactggaaactttggtt
ctctgcgtctctatacagttgtcagtggattggagggaactgttgttcagtaactggtccaggataaaagtaaatgttccctactatggctgacattatg
aaagaatgaaaacccttgagaaaatttatagctggggctgatgccacctctatttcacttacattcaaaggctggggttg
3' EST: CX967674 [+]aaaacttacagcaggcaaatttttaaattcaatgaaactgttgcttctactcctgttcacaagaaaggctcttggttaaggtttctattcaaagaaaaat
gtactctgatgttaattggaaattttctttgattcctaaaacataaattttctgcataagaggaactttgatattgctttctttatgaggatactttcct
tagacaacattagaccaatcactgttgtgttatgaccgctatatgtggttccactgaaagtgacatggtgtctcccatcatgatgggcatttccagtgcc
ataactacatattactgggccccacagcaaattcagtgtagggccccagacattggtgagttaactattctactaaggtctattgaagtcactcattact
tagagccctactaagccctctacactcttgcagggtctgcaagacagcccccaccctaggcacatgcttcttctgcctacccttagttctgccctggttc
atatccagaacaacaccactgtatgacccacactgtgacctctgtaccacatgtgagcacccaggcttcaaccccccagcttttgaattgtaagttgaat
aagaggtggcatcagccccagctataaattttctcaagggttttcattctttcataatgtcagccatagtagggaacatttacttttatcctggaccagt
tactgaacaacagttccctccaatccactgacaactgtatagagacgcagagaaccaaagtttccagttctacaatcctggaacttgtcatgcctggaaa
cagaagcagtaaggaaggggtttgttcaggaatttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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