XB-CLONE-3561325
Clone Name: IMAGE:7711286
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967689 [+]
caaccgtctgtttatgaagtgtcgaccttttcctgatggtcttgctgaagacattgataaaggtgatgtatctgctcgccaggagttgaagactcgtgcc
cgctacttggctgaaaagtacgaatgggatgtgactgaagctaggaagatttggtgttttggtcctgatggctctgggcccaacatacttactgatgtta
ctaagggagttcagtatctgaatgaaatcaaagacagcgttgttgcaggattccagtgggctacaaaggagggtgtcctgtgtgaagaaaatttgagagg
tgttcgttttgatgtccatgatgtaacacttcatgctgatgccatccatagaggaggaggacagatcattccaactgcacgccgcgtcttgtatgcttgt
gtgttgactgctcagccacgtctgatggaaccaatctaccttgttgaaattcagtgccctgagcaggtggtaggaggtatctatggtgtcttgaacagaa
agcgtgggcatgtctttgaagaatcccaggtggctggaacaccaatgtttgtggttaaggcatacctgcctgtaaatgagtcatttggtttcactgctga
cttaagatcaaacactggtggccaggccttccctcagtgtgtgtttgaccactggcaaattctacctggagatcctttcgataactccaccaggcctttc
caagtggtggcagagacccggaagaggaagggcttgaaagagggtgttncagccttggataacttccttgacaagctgtaaacctttgctttactcctgt
cagcattgattaagtggaaatgaactatacccaaaagatgtcagc
3' EST: CX967688 [+]tttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttacttc
cccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttccaac
agaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaaggaag
ttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccaggta
gaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcaggta
tgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacctgc
tcagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatgatct
gtcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcaaattttcttcacacaggacaccctcctttgt
agcccactggaatccctgcacaacgctgtctttgatttcattcagatactgaactccct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: