XB-CLONE-3561249
Clone Name: IMAGE:7711246
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Gene:
c6h6orf62
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967743 [+]
ctagatacaattttaaaccaggtaagcactagtattagccaaagggaataggcctatacaagttgattcataaaaggtactgaaaccttttagaaaagcc
ttctcttaaacaatacagtagtcaaccttatcattgcacaaaataaaatgattctttgaaccaaatttcacacttcaaacttcttttacaatttgtaacg
gtgttctgacattactttattgaaaccaccaagggtttttcttttttctatagaaatattacttcattttatgaaaaagtaatatttgcacaaatcacca
gcaggtttctatttcagttcctatcttcagaaacatggtttctgaggtgtcaacgaattttaagtcgttttcatatagttactgcagactcctagactta
tgtcagtacccacaggtaggggaactctgttatgcagcaactgggagaatataccaaagtgtaaaagtgccattactcgttcactgccctgcaagctgaa
ctacgatatgaaagcaaatgctgaataaagtttctttttcaatacatctgagccagtcttcattgtaagccaacatagggtatgctttaagagattcaga
tagtgctttcataatacagtgaagcagcaaagctatttatacaacttacattcattttttttcaccctcaatactgtcatctaaatgatgttaggggaat
gaccagtgcaaatgggaagaccactaaacaaaataccttgtcatttcagatacatttgattcagtgtgccacagtcccatcaattcacattttgagaatc
tataacaaagatgtattaaacatcctctcttttcacaag
3' EST: CX967742 [+]ctttggcagggagacttgacatgcctgtggaggatgggttttgagtttatggtacaggttagtggcagggaataaatttatatttttgtttcaatattat
accctggtgttttaactggtttgtctttttatgtttaaaaaaaatgcctcttttagttccatcagaaaacaccacccagtatgtcatggctgtacagccc
tttatatttcctgaccctaatagtaaggcttaacacacattgtctgaaaagtgctttgggttgttctgcccagttgatttaatcactgaaaaggttgtaa
catggcttgtttctgctgaactggtgtcctaaacgcacaaaaattgcccgcaattagcaacaagctttctaagcgtttgcaccagctgttcaaaacaaaa
tatgggggacccaaactctcgcaagaagcaggctcttaacagactgcgctctcagctacggaagaagaaagagtctttagctgaccagtttgaattcaag
atgtacatcgcctttgtgttcaaggacaagaaaaagaagtcagctttatttgaggtggctgacgtgattccagttatgaccaataactatgaagagaata
ttctgaaaggtgtgcgagattccagctattccctggaaagttcccttgagcttctacagaaggatatagtacagcttcatgcacctcgctaccagtccat
gcgcagggatgttattggatgcactcaagagatggactttattctttggccaaggaacgacatcgagaagattgtctgtcttcttttttcaaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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