XB-CLONE-3561200
Clone Name: IMAGE:7711108
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Gene:
cd74
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967779 [+]
ccaagagcaagctatggcagaagagacccagaacttggtgccggagcatgttcccgaagaaagtgtggtcgatgtcggaaacagacagccacggatgtcc
tgtaacaaagggagcctggtgacagctctaacggtgcttgtagccgtgcttgttgccggacaagccgttatggcctatttcatcacccagcagaattcca
agattcagaaacttgaccaaaccaccaaacacttgcagctgaaagatatgatgaagaatcttccaggatcgcctcctgcccaaattaaatcaaagatgag
aaccttcaatatccccatggcgttaaaactatatgatggctcagaaacaaatatgaatgaactggagcatctggctcagatcgataacaaagtggaagat
gctgcaaaatacatgttgctgagaggaaaccctctaaggaagtacactttaccaaatggcactatcctggaaaacctgagagagctgaagaagtccttga
ctgaccaggaatggatgaactttgatgcatggatgcatcagtggtatctcttcttcttggtgcaaaacactggaaaggcagctgaacccctcccaccgca
aaagaatattgcagtcacaggtgcccccctgatgactgagtgtcagacattatcaaggatccccaccctgactggcgcttataagcctcagtgtgagcaa
aatggtgactttaagccgaggcaatgctggccaagcactggctactgctggtgtgtttaccgtaatggaactgaggtccctgactccaga
3' EST: CX967778 [+]taagatgtaactgctcattttattaggcatggaataaggtatgtgcacgttatacagaacctgtaagtctgacagaagccgcaattaacagcagagaaaa
agttaaaaaaaaaataaaaatggcagccatatttctatcaaaacacaattagcgataaatgaatgaacaacgattttatcagtgcccatttgaattgtca
aatcatgttaatgttatcacattccccagaccgtaccttactgagacctccttaaccatacagaaactactagatttgttagtagtggcaagacgtggag
gatttgtccatggtatagagacatagacaagccacttttcagaagtactagttagtcagcctcctggtagatacttcctttcaatcatgtgcagctacaa
tcacagtgggactgatctgtgtaaccataccacttattattcctttaacatgctgctgtaacacacactgtaattcataaaaacaaacaaagtactaaac
actaccttaagcctattgagaaacctgaggccagaatgccatagcaattagtaacaacataaagtcagattaataggaatcctcttgtgctgcttgtatg
aatcagggtagatactgcagaggaggttacgtgctgatccgatgctttaatcaatgggattaaagtcatcgctgggtgttgatcccagaaaaattggttc
ttctggctccatgacatcactgcaggcaggtcttgaccaccgggttctggagtcagggacctcagttccattacggtaaacacaccagcagtagccagtg
cttggccagcattgcctcggcttaaagtcaccattttgctcacactgaggcttataagcgccagtcagggtggggatccttgataatgtctgacactcag
tcatcaggggggcacctgtgactgcaatattcttttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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