XB-CLONE-3561175
Clone Name: IMAGE:7711158
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967797 [+]
aagattggttacaaccctgatactgttgcctttgtacctatttctggatggaacggtgacaacatgcttgagcccagcgccaatatgccttggtttaagg
ggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctggaagctcttgactgcattttgccaccaagtcgcccaactgataagcctct
gcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtaccagttggtcgtgtggagactggtgtccttaagccaggcatggtggttact
tttgcccctgttaatgtaacaactgaagttaaatctgttgaaatgcaccatgaagcccttagcgaggccatgcccggtgacaatgttggctttaacgtga
aaaacgtttctgtgaaggacgtccgtcgtggcaacgttgctggtgacagcaagaatgacccaccaatggaagctggtacctttacagcacaggttatcat
cctgaaccacccaggccagattggtgctggatatgcccctgtgttggattgccacacagctcacattgcttgcaagtttgctgagctgaaggaaaagatt
gatcgccgttctggtaagaaactggaagacaatcccaagttcctgaagtctggtgatgctgccattgttgacatgatcccaggaaagcctatgtgcgtgg
agagcttctctgactacccccctcttggtcgttttgctgtccgtgacatgaggcagactgttgctgtaggagtcatcaaggcggtcgat
3' EST: CX967796 [+]aatttggtgaagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaaaaactgccacaaacaaatgtgtccacgagc
atttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaattgatagaaagagttctgagacca
atcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactttgccagctccagc
agccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtcagagaagctctcc
acgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttaccagaacggcgatcaa
tcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctggcctgggtggttcaggat
gataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtccttcacagaaacgtttttc
acgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgttacattaacaggggcaaaag
taaccaccatgcctggcttaaggacaccagtctccacacgaccaactggtacagtaccaataccgccaattttgtagacatcctgcagaggcagacgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: