XB-CLONE-3561172
Clone Name: IMAGE:7711206
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Gene:
tagln
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967799 [+]
tattgagaagaagtatgatgaggaactagagcagcgtctagtgcagtggatctgtattcagtgcggtgatgatgtaggaacaccagaacaaggaaggcta
ggctttcaacaatggctgaagaatggcctggttctttcaaagctgataaacagtctgtacccaaagggatcccagccagtgaagatccctgatccccctc
ccagcatggtgtttaagcagatggaacaagtggctcagttcctgagggcatctgaagattatggggtggttaaaacagacatgttccagactgtagacct
gtatgaaggaaaggacatggcggctgtgcaaagaacaattgtggcacttggaagcattgctgttacaaagaatgatggccaatacaaaggggacccatct
tggtttatgaagaaagcccaggaacacaaacgggacttctctgatgagaaactgaaggaaggaaagaatatcattggcttgcagatgggtagcaaccaag
gcgccacccagtctgggatgacaggatatggtcgcccaagacaaattatcagctaaaaaaaaaaattggcataattaacaactataacctctttgctgcc
aaaaatacaaattctacacctagaagcaaagaggttaatgcatacagtaatacatataaggcctcaccatgtacctaatcagaatgaaaaataatccaag
ttctccagtaacaggtattaggctggggctttgaagggtttaatctgtcaggtcaccttgaaaaactgtatgcaaaaactgtctacaanatgtttagtac
attagtttttaaacctctttggggacttaccaatagtggatatgca
3' EST: CX967798 [+]atgatcatccaacagtgtacaaaaatgagttttgttttctcattacattacgagtctcattaaacttactgctggattttaatgcaaacatcaaatattt
aaacttattattgcatattggtgtaaaatacacctttaagtgccagtagatgtacagaatggtttcttttttatttagaatgtctaaaaaaaaaaaacac
atgaaccccattagcagacctagcggggatgtattgtgtaattatctgaaaaccaagtttcacattcatactgccctctgattggacatgcaactgtatc
acttaacaagcatcaacatcatgtttctggttggcttcacagcttgctccaatcacattgccatgaccaggaagcttgatgtgtgctgggaagcctgggt
gggaaggagctaacctagcactactcactactaccaagacaatgttaattgtaaaggtattttacaacaaatcacaactaataaagtttatttttgtaat
tctgttggtttccaattgactaatttcagatttaggacacactagacattttattgcagtgatcaaacgtcccttaatctctgccaactaattcccataa
aagtgtatggggaaaaaactgttggatcaagagtattatctgaaatcctagtgattataatctgatgtctgggttctttggtgatgtgcactttggagtg
gcattagcagtgggattcaatacttgtacaatctgttacctgtgggttaaggtatgtcagattgttgtagttttcattgatgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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