XB-CLONE-3561148
Clone Name: IMAGE:7711208
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Gene:
amacr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967815 [+]
agctggggttcgtgttctggaactggcaggtcttgctcctgcgccattttgcggaatgattctagcagattttggtgccaaagtgatccgagtggataaa
agtagtagcaatgtaataatggacaccatggcacgaggaaaaaaatctatagttgtgaacctcaagaaccgggaagggatcagtgtattgaagaaattat
gcaaaaagtctgatgtcttgattgagcctttccgtcatggtgtcatggagagccttgggctcggacccgatattatgcttaaagaaaacccacagcttat
ttacgcaagactcactggatttggccagagtggaaaatatgcaaaatcagctggtcatgacatcaattatgtctcaatgtcaggtttgctgtcaaaactg
ggagtcaaaaatgacagtccaaccccacctcttaaccttctggcagacttcgctggtggtggtctgatatgtgcattgggaattgttatgtcgctgtatg
agcgcactatgtcaggcaaaggccaagtcattgattgtagtatggtggaaggcgctgcatatgttggatcctttatttggaagagccagaaactgggcct
gtggagtaattctccaggtgaaaatttgctggatgggggagctcctttctataccacatacatgacggcagatggaaaatttatggcagtgggccctctt
gaaaccaagttctatgccgaattgttaaaaggacttgagcttgatgcctctgaattacctcatcagatgagcttctcggactggcccgaaatgaagagaa
tctttgcccaa
3' EST: CX967814 [+]tgttacaatatcccattaaaacacagaatatttaaatcttgcataccaaacaccacagccttctcttatgtctttggacacattatagaacaaaatccag
taatgaattagatgctgattgtcggcaatcagtaatttttgatatcacaatttcagcttgcattgttaaccttgatactgttctttacaactgagctttg
gctttgtttgttgcaactgctccgcgggtttggagatcagaaatttccctttggctgaagccatattctgcgagtatctcatgcgtgtgttctccgacca
aaggatccctgctggtgtttggagctgcaggtgttctagagagaagaggagcaggccgtgggcttaattccccttcatcgttgcatataaaagatccacg
atcctcattgtgttggtgtaaggcaacgtcactaaagctgaggactggagtaacacaagcatcggttccatcaaatattcggcaccattctgcctgtgtt
ttctctcggaacttttgggcaaagattctcttcatttcgggccagtccgagaagctcatctgatgaggtaattcagaggcatcaagctcaagtcctttta
acaattcggcatagaacttggtttcaagagggcccactgccataaattttccatctgccgtcatgtatgtggtatagaaaggagctcccccatccagcaa
attttcacctggagaattactccacaggcccagtttctggctcttccaaataaaggatccaacatatgcagcgccttccaccatactacaatcaatgact
tggcctttgcctgacatagtgcgctcatacagcgacataacaattcccaatgcacatatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: