XB-CLONE-3561130
Clone Name: IMAGE:7711114
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Gene:
ift46
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967827 [+]
cggcaggggagatttgtcgcatggcgactagtctcccctctgtcacaccccttaaaagaggactcagtgcagcttttctgtgagtgcttatagctgtaat
tacatagatctttctgataaagcttacttagtttttacctttccttctcttttaagcctttcatatgtaaatactaaattaatctgcttgagatgatatg
attaaaatccctcccattaaatcatactaaccttcgcctaatccaactcttcctagacccttacaaacagaaattcactctctaccctcccatttactcc
ctcactgcttccactagggttccaaaccaatggaaaaaagacaaaatatctagatatagtgtgggctgtgtatctcacttgcagcttaatagccagaaac
agtgaaaggtgaacttaaggggagagacagagagatgggtgaataagagaactgaggaggaaacagtgtaaaaaatatgtaggggagaaaaagtggacag
taaaaaaaagcaaatgaaatagaaaaagggttaagtaaaaaacaggacagcagaaagcaagagcagaagagaaaggaaaaaaggctagatgagcacatga
aaagaaatctaaaagtgaaaataggaatatttatttacattgcctttttacaatgcttttaccaaagctgattttcacaactttacactagcttagactt
tttaaaacgctgcaggaggtttgcgacgatcaaaaaggtgtaggcaccattacccttgcgaagacattacttttctaancaggaatcataagaattaata
acattttcagtagtggagtaaaactttactaanactgcactacaagttatatttca
3' EST: CX967826 [+]aaagataagtttgcacatacagagatgcaaatatgctgtactaacgtttggtgcccagtcctcccgaaacagcagagcaggcaataatgcagtgtaaagg
atacgaccatcaggatacattgttttaatttgcctgaaatataacttgtagtgcagttttagtaaagttttactccactactgaaaatgttattaattct
tatgattccttgttagaaaagtaatgtcttcgcaagggtaatggtgcctacacctttttgatcgtcgcaaacctcctgcagcgttttaaaaagtctaagc
tagtgtaaagttgtgaaaatcagctttggtaaaagcattgtaaaaaggcaatgtaaataaatattcctattttcacttttagatttcttttcatgtgctc
atctagccttttttcctttctcttctgctcttgctttctgctgtcctgttttttacttaaccctttttctatttcatttgcttttttttactgtccactt
tttctcccctacatattttttacactgtttcctcctcagttctcttattcacccatctctctgtctctccccttaagttcacctttcactgtttctggct
attaagctgcaagtgagatacacagcccacactatatctagatattttgtcttttttccattggtttggaaccctagtggaagcagtgagggagtaaatg
ggagggtagagagtgaatttctgtttgtaagggtctaggaagagttggattaggcgaaggttagtatgatttaatgggagggattttaatcatatcatct
ncagcagattaatttagtatttacatatgaaaggcttaaaagagaaggaaaggtaaaaactaagtaagctttatcagaaagatctatgtaattacagcta
taagcact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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