XB-CLONE-3561081
Clone Name: IMAGE:7711214
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Gene:
lims1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967859 [+]
aggcttaagaagctatctgaacccctagctagaaaataagtgtgtcttaccgacagctgcagggtgaagacttggaggagcatgctacttgactttgtcc
tgttcactgaggctgttatctgcactaaccttcacagcaaaatatgtatctgcatgaatactgcattaaactaccacatgtgctatttatcctatcacct
catccttaaagagaaaatactctgttgctcttgtgctacctgcctcaacttgttctgttagcaatcgctcttcgtcaataactgagcggggctgggacaa
taatatttctaaccatcattgctgctattgatattgtaactgggagaccttttgtttgaacctaaatgctcatttggtgttttaatttgagatactgaag
ctacacagcagcttagcttcagagtatcccttatagatctgatttggaagtattttcatggaaatcagtgttaccttaggtgagttggacttgtgcacca
acccactgaaaaaattcttgagaagctgtatccacagaatgtcaaaccatatcaattgtgtgatggtacagttgttcttctaatgttacatatgcccttt
ctagctttatcacaagtagaacctttcggccttcatttgtgccaaacacattgctttggctcacactgaagtaaccagtaatctttaacttattggtctc
cagaatgtacaactcccagcatccttcagcaggtggaagatctcttacctaaggtctgtaccttgcattatataatgttcttgcatttcaagcaggcttt
agtatagcattgtttacacatggccaagctatatggccactgaaatgacagtaatgcatgcctgacacaaaagcatctatttcatctgtatgcatactca
gctttgtttctgcaaca
3' EST: CX967858 [+]gagggccaacactgtttatttaaaggataagattatagactgttatattcccataaaacattttttttaagaaacatatggtaaaaccttcaggcttatt
catatattacagctaactgggcaacacaaatgtattctacacagtgtagaataggatttgttagatataaattcatgaatatgtaaccataactatgatt
ataacttgcaataaaaatacttcatatattcaagataaaaacctgccttctgggattaacagcatgggtttactgcatagattagaaaaaaaagcttcca
tatgtataatattaaatatgcaatatacactacgaggtctgttagattgaaataatacagataaggcacacgaaaatgaagcagtcaggataaatgagac
atgcacattaagaaacatgctgcagatgagtgtaattcaagtagttagatcagcaatattttgcaaagtctgtattctattgttccctttaaagatatta
cactaccagagatgtctctgtgctccacattacgtgggtaaaggcactggttctgcattggttgtgcaagtaagttgttacaattaaacacgatgcttct
ttaataagtgcgtgagtggcactcgtactgcctactgttatttggaatgtgggcaattcagcccttgtttgcataacttctgtgggctagagcataatat
acatatatatatatatatatatatatatatatacatatatatatatatacatatatatatacatatatatatatatacatatatatacacgttctatata
tttatataaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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