XB-CLONE-3561064
Clone Name: IMAGE:7711168
|
Gene:
ccdc6
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX967870 [+]
agccatgcagacctgttccccggcttcttcatcttcgggcaagtccggcatcgtcatctcgcccttccgcctggaggagctcactaaccggctggcctcc
ctgcagcaggagaacaaggtgctgaagatcgagctggagacgtacaagctgaagtgcaaagccctgcaggaggagaaccgcgacctgcgcaaagccagcg
tcacaattcaagcgcgggcagagcaggaagaggagttcatcagcaatacgctgtttaaaaagatccaagctttgcagaaagagaaggaaaccctcgccgt
caactatgagaaagaagaggagttcctcactaacgaactgtcccgcaaactcatgcagttgcagcacgaaaaggctgagctggagcagcacctggagcag
gagcaggagttccaagtcaacaagcttatgaagaagattaagaaattagagaatgataccatctccaagcagctcaccctggagcagctgaggcgggaga
agattgacctggagaacacactagaacaggagcaagaggccctggtgaaccgtctctggaagcgaatggacaagctggaggctgagaaaaggatattaca
ggagaaattagaccaacctgtttctgctccaccgtcgccacgagacatctcaatggaaatcgactccccggaaaacatgatgagacacattcgatttctg
aaaaatgaagtagagaggctgaagaaacagctgagggccgcgcaactgcagcacactgagaagatggcgcagtatttggaggaggagcgccacatgaggg
aggagaaatttaggctt
3' EST: CX967869 [+]tttgaccatcatgcgttttaatttttacgcagaccaacgaaggggaacttaaaaaattaaaaagaaaaccccaaggtatccctttgattacacaaaccta
aaagcgtcggagcagcgcgacttaacatggtttgttctactcaataccaagtagcgagtgttccagccgaggatgagcgcacatgtagttttctccttaa
acatggacggtgggggatacgtgttgggaaggtaacgctgtagacccacaacactggctgcgtttcgcagacaaacatttacaacacgattaaacagtga
agcctatggaacctcagtgcaaatagtcaaaatcgaaatatgacagggaagcctttggctttcggtaaactgtcccacgttatgagtccaactcaaaagg
cagagactgcagaggtcatgcactagggctgagaggaggggtgcactgaattgtgggaggctgaggactgagacgctgcaggctgcgctggaggaggcgg
tggcggcggaggttgaactggagtctgggtgttgggagatggaggtggcgtagggcgcttgaacttgtctgggctgttgcttcttcgaggagaaggtctc
gtgataccgtgagatgatcccatatgttgcacgtgtaaacctggcgagttaaagtacgacattccggctctggtcagagaggttgggggcgtgaagccca
cattgtgacttgcgtacgacaggccgggtgatatcggcctgctggaacttggcgatggcgtatacggtattgggctggacacagtgcgaggcctcaggcc
ttgtgccgacatctcattaaaatacctttcatcatccatttctaagctggactcgctttctgataactgtcggcaaagagcttcccgcgctccatctccc
gctgcaacttcctctgaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: