XB-CLONE-3561042
Clone Name: IMAGE:7711218
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Gene:
faxdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967886 [+]
caaaattagaacataattgtggcacacgagttagttgtgattagtgtaagaatatgatgctgttgcacccacccaatcccactcgtcactactgaataat
gtacatacggtaacatccgtacctaatgggccactatggagaacagtgatgtcagtgttctcaatggaccctaaatctacagctaaatatccacagacac
tgggttttttttctatgcacctacagccatgaacaatttctaaccttctaattatcatttaatcacagagataattagtatcataagtcagtcaaaaact
ggagtgggtgccccaacacacccaaacccgctggatgttgctagtacattagcacaacattaagggcaaaccatatccttgcaggtctctgttcaagagg
gacaacttctcttctaacatttccccctttagactattgggactttttaggggtgtcaggaatgctctgacttagtggtgtaaggttcattaatagaatg
tgtctttcataagccttggtttgcttgaacatcagatctgttccatgcagatgatccaataccccaagtactccgtagcactggttaaacttgagatggt
gaaaatcatggaactctggagaaggcaggaaaggcaggtggtagccacagtgtgagatggtggtggtgatcaaagccaggcagaaccacagcatggttgt
ggccacgtgggatcccattaccatagggccgaccatggatggcagcatgttggaaaaaatatgctccaggggatgtgcatacaggcacaccactcctaca
ggtgccgtccattcatggtgcttcttgtggatgcgcttgtac
3' EST: CX967885 [+]ctaacccgttacaggtaccatgctgatgcccacgctttccttctgggtcttcaatgccatgctgatgcttatcgacctcactggaaagccatacttcatc
accaaataccggatccagcctgggaagaacgaccccgtgcgtatgctgcgctctcatgttatgttactgttctctctaaatgttcccttgttaaagctaa
aattatacctgattaaaggggaacccacccaaacacaactaagttttttgaaaagtaaacagaatttcaagcaactttgtaatatacatcaattaaagaa
tatgcagccttttcattatttttaatgtaataatatggattggaacagttccataagccccaccccctgttcccctgctgatctgactgactgcattgag
actcagataaaatataacagtagtcgcctgtctttagcctgccttcagcctgcatcctcccaatcccacaactccctgcacacgtgatgttaataatgaa
aggaacatcacagggcaatgcgttgtgggttatgtagttcctgcagtctgtaagctgtggatactttgttacaatttgtaacatcagtgtttaatcccct
cctcccctgccaggatttcaaatcctgtgtatttagccaaagcccagacatactccgtcagtattcatttattttattgtgcacccactgtacctctcat
ctccaggtggatccagccaagctccgacaggccgtgatcactgtgcttgccaatcaggtctttctctcctttcccatgatcgttctcatgtatcccatca
tgctgtggcgaggaaatccctgtggccctgagctgcctacattccattgggtccttttggaactcacagtcttcgcgctgggtgaggaaattctctttta
ttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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