XB-CLONE-3561028
Clone Name: IMAGE:7711076
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Gene:
siglecl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967895 [+]
ctatgactgtaggcccatctgtactggctgtctatcaggatggggggcagaagatcttggttttactggaaataccatattgttctgtctattttgtcag
tgcagtggaagggaatgaactgtcaggagtctgtaccagggtttaaaatagaggccccagcaggggtgagagtgcagagggggctctgtgtgcacatccc
ttgtagctttactgttggtcctggttttaccttaaccagagatgccattggaatctggtataaaggatataatggatttcaaagtgggataattgcagct
tctactgactcctctcagttccctgatacaacaaacgggcgctttatattcactgggaaagtgagtgccggagactgttccttctctatcagtgatgcac
aaccaggagatgctgatcggtaccagttccgacttgagggtcctctaaaatataactacgttggtacccaaccatctgtatctgtgacagatcttgcaga
cctgaaagaacctgatatatctcccaccgaggatctgatcgctggggaagaagtgactctgacttgcactgctcctacaaactgccctgggctctctcca
accttcacatgggaagggagtgtaaatacagaaagaacacagaaccatacattgcagcaccaagatgggaacttcagctactggtccaatatcactttca
ctccatcaccaaggcaccataattcccccttaacctgtacatttacctataagcatgggtctgctaaggcaaacatcaccctgaatgttgaatatacgcc
ccttctgaacatcactattc
3' EST: CX967894 [+]acaagatataaaagtttattgatattaaacatgatttttaggactgatacattcttggctggttgtccaaacagtgatttgctcttattgtataactaaa
aaatactggcaaaagtagtttttctcagaacaggtatgggacctgttatccagacaacttgggatctggggttttccagatatcactcatacctcttcat
acaaatttgaataatcgttattccatttggttttctccgtgtccatgtaggattcattgttgatgctttgtatattctctacgtcattgtaaatcacatc
accatcactcttaggcatctctttagtgtcctcctgtacgttcttcttctttctcatccatatgaatacaagcaccgccgctatcaccgcaagtgtgaaa
aataaaatacaaagtcccacaatgaggaaaactgtctgagacttttgtacaggcgtgacaataataaggatggaactatttgatagtccgtatctatttg
tagcttggcatgtatatacaatatcatttgtaacctggacctttagaatactgttttcagaaaatgtgttgagtggatcattctgaatccaggaaataat
tgcctcagggttactttttgctctgcagtttaaggacagtgtcgatccttctggaacagagtcatttactaaagaaaggcaaccttccggcttatctaca
cagatcgttggctgtcgggggggatattccacagagacactgacagagcggctgttgctccccctttggttctgtgccgtacatgtatatacagcggtgt
cattctctggtacattct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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