XB-CLONE-3560950
Clone Name: IMAGE:7711322
|
Gene:
ybx1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX967947 [+]
cgcaatggttacggcttcctttaaggaatgacaccaaggaagatgtgtttgtagaccaaactgccatcaagaagaataaccctaggaagtaccttcgcag
tgtgggagatggtgaaactgttgagtttgatgtagtggagggtgaaaagggtgcagaggcagctaatgtaactggtccagggggtgttccagtccaaggc
agcaaatatgcagcagaccgtaatcatttcaggcgctatcagcgtcgcagaggtcctccacgtaactaccagcaaaattaccaaaataatgaaagtggag
agaagacagaagggaatgagagtgcacctgaaggagaggggggttcaaatcagcaacgtccataccgcaggagacgtgttccaccatactacttgcggag
accatatgggcgcagaccacaatactcaaatgctcctattcagggagaaggtgcagagggatcagatggtcaaggttcttcacaaggtgatcaaggtaga
cctgtgcgacagaatatgtacagaggcttcagaccacgatttcgcaggggaccaccacggcagagacaacccagagaagaaggaaatgaagaggacaaag
aaaatcagggggatgaaacccagagtcagcaggcacctcaacgtcggtatcgccgtaactttaactacagacgcagacgcccagaaaaccctaaaccaca
agatggtaaagagaccaaggcagccgaaacatcagctgagaacacgtccgctcccgaggctgagcagggcggggctgagtaaataccggcttaccaactc
taccaccatcgggttttttgtcatcaaaatgtcaagacagaaat
3' EST: CX967946 [+]aaaaaattaggtgtacgcttattacaaattaaaaatgaggttcttaaaaatcttagtttgaccagataggaaacaatttaaaaacctttgaaaggtacat
tgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagcttttagaaaaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagt
tatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaattctttgttcatttcttattgctggaatttctgtcttgacattttgatgacaa
aaaaccggatggtggtagagttggtaagccggtatttactcagccccgccctgctcagcctcgggagcggacgtgttctcagctgatgtttcggctgcct
tggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtctgtagttaaagttacggcgataccgacgttgaggtgcctgctgactctgggt
ttcatccccctgattttctttgtcctcttcatttccttcttctctgggttgtctctgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtac
atattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttgaccatctgatccctctgcaccttctccctgaataggagcatttgagtattgtg
gtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcctgcggtatggacgttgctgatttgaaccccctctccttcaggtgcactctca
ttcccttctgtcttctctccactttcatta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: