XB-CLONE-3560921
Clone Name: IMAGE:7711469
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Gene:
shmt2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967969 [+]
cacagcccctgcggagttgctgccatgttcgcagtcagcacagccaagcttggacacaggctggaaaccaagtctggactggacaggaaagtctggcaga
gggtgaccctgagatgtgggaccttgtacagaaagagaaggataggcaatgccgagggctggagatgattgcctcagagaatttctgtagccgggcagcc
ttggaagcccttggttcctgcctcaacaacaagtattctgagggctacccagggaagagatattatgggggtgcagaggttgtggacaagattgaattac
tgtgccagcagcgggctttggatgcctttgacttgaatccagaaaaatggggagttaatgtacagccatattcaggctctccagccaactttgcagcata
cacagctgttctacagccacacgatcgcatcatggggctagaccttcctgatggaggacatctgacccacgggtacatgtctgatgtgaagcgcatttct
gctacatctatatactttgaatccatgccttataagttaaatccagcaacagggttaattgattatgatcagctggagatgactgctcgcctcttccgac
caaagcttatcattgctggaaccagtgcctatgcacggctaattgactatgccaggatgagaaaggtatgtgatgaaatgaaagcctaccttctggctga
tatggctcatatcagtggacttgtagcagctggcgtgatcccatctccctttgaacatgctgatattgtcacctcaacaacacacaagactttgcgaggg
gcaggtctggggtaatattctacc
3' EST: CX967968 [+]cactgtaccatgatgtttttgtttattgctctaacagtctctggagtctacctgaatatgctctgctgcagcatgaatccccttaccccacactgaaatg
tcagatttggagttttgagatttcattatggaagtccaatgcaccacggaaatgacaatgaaacaaatctagctgcctagttcttcatgcagtgatgctg
ctggagagaaggtttgccaagtagaaaagggtcctgggtcactccgttataagaaaagataagagacaactaaatatatgatgcaggaatagaatgcaat
aagaaattggctggattaacagaaaatgcaatgaggcaacaggaagcgcaggaagaaatagggtggaatggtttgttgttcttaacaccatcaatcatct
ttttattttattcaacgctcatcaaatccaggcatgggaaaagaacgggcaaactgctccacttgcttacgcaggtctgctatccgattaactgtttcgt
ggtcctcaagaagaaagttcttaaagtcttgaagtttgtttgttttgcgcttaacatcaagtccgatgcggataccttcatcaatgaaatgaaccacttt
ttcaaagtcggcttccttgaaattccgagatgtcagcgcaggggcacctagccgtaaccctcctggggtaagtgcgctcttgtcccccgggcatgtgttc
ttgtttgcggttattgacacaagctccagcaccctctctgctcttgcgccatcaatgcccttgggtctcaggtctactaaaaccaggtgattatctgtcc
cctctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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