XB-CLONE-3560740
Clone Name: IMAGE:7711629
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Gene:
capn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968092 [+]
ccatatgtaacatttttttccttggttatctactggcagtaatgacagaaacagacaggagcagtgaataccgatactgcccccccaccttctggtattc
ccacatacccaaatggagacagtgagcacttgtattgctaccactagaaacttgggtgcaccctccctcagtcaagagggtttaggcacaactttatcaa
gcattttgacacaacaagggcagattttggggtagggcaggttagggtcgggcactggtcaataatctattgaccagcacatcactagtcagtgcctaag
tgtacaaaagaatagcattcagagtaattgtatatgtagcacattaagtacaatctttttcagtggcctaaaaaaatgtggtgccaagaaacgcagaatt
acaggatccaagataatccctcatagtcttacattggtcttttttgcaatggaaatatacacagagcttgttaatcggtacttaaaaatgcagcacttca
agcacctcaagaacccccatgttccatttaaatgtcacaagagaggtttctatgccagctaagaagtgctgaatatgggttgtatcattttccattagct
ttaaaggggtgtttcacctacaaattaatttttttagtatgttatagaatggcatattctttgaaacattccaattggtcttcattctttatttgttata
gtttttgaatgatttgccttattcttctgactctttctaactttcaaatgggggtcactgaccccagcagtcaaaaaattattgctctgcaaggttacaa
atttattgtcattgttacttttttactacttac
3' EST: CX968091 [+]ggcgagaggcttgcaccttactttgactaaaaaacagtctgtcaacggtggtacagaccgtttttagctccaatgcacattttttggcctgtcagtgtgg
tttctttttaacgggtagttcacctttgaattaagttttagtatgatgtagacagtgctactttaaaataatttttgaattattgagctttcagcagatc
tccagattggaatttatgcagctatctgattgctagggtccaatgtaaccgagcaaccaggcgttggtttgaaagagagacaggaatatgaaaagaggag
ggcctaaatagaaaaataccgtaagtagtaaaaaagtaacaatgacaataaatttgtaaccttgcagagcaataattttttgactgctggggtcagtgac
ccccatttgaaagttagaaagagtcagaagaataaggcaaatcattcaaaaactataacaaataaagaatgaagaccaattggaatgtttcaaagaatat
gccattctataacatactaaaaaaattaatttgtaggtgaaacacccctttaaagctaatggaaaatgatacaacccatattcagcacttcttagctggc
atagaaacctctcttgtgacatttaaatggaacatgggggttcttgaggtgcttgaagtgctgcatttttaagtaccgattaacaagctctgtgtatatt
tccattgcaaaaaagaccaatgtaagactatgagggattatcttggatcctgtaattctgcgtttcttggcaccacatttttttaggccactgaaaaaga
ttgtacttaatgtgctacatatacaattactctgaatgctattcttttgtacacttaggcactgactagtgatgtgctggtca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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