XB-CLONE-3560728
Clone Name: IMAGE:7711439
|
Gene:
tmbim1
,
p3h3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX968100 [+]
ttaaggctttgcctggactcccagtcacttggtacaaaggaaatatttacatataatgtacattctgtcccacgctctccgactgattataaaacccaca
ggcagggggcgcttggttaagaatacagcgcccaaactggctgcatggaaggccaaatcctctatggataaagacattacggaggcgccattggcatttc
agtgcactttaacacctgccccattagctgagaattaataggaatattctggtctggttccatgatccatgggagacggtatttctgcttagtattaggg
tatagtaacccccaccccatccctttaataaaccacttacagaccagaatctctctgttcaagcactaatggggtgaccccagaattaagacttgctgtg
gggagggtgggatgcaaagtgcctgttaaagaacagaaagggactagaattctacttgcgtccgtgtaacagggcagcacctcgtatgcacatataacag
taaacaaagtcagtcctcgggccccagaacagcgctttatattcctcccctggcagatcaatccgtgggcctagaactctcacatataaaacagtattgg
ggccacaggtacccagccagggtggtatgtgagctactggcagaggtcccccaagacaaactgtatcagaggaatatatagtaaacatacagtatataca
gagtgcaaggcttggcttatgccattaaacagcagctgattttaaaggngaagtttaacttanattgtacttttagctaggcggcttttcagtttggcaa
taaaaccaacagacccaggcaataagggcatttctctaccaaaa
3' EST: CX968099 [+]gaggtgacggagagggtggaaattttggttcgtgggatgacaaggccgtgcgtcatgcctttatccggagggtctacgccatcatagctgtgcagttgct
gctcactgttggcatcattgcgatcttcacttacgtggaaccagtcacctcgtttattcgaagaacacccggtgtttactatgcatcatacgctgtgttc
tttgtcacatacatcgtgcttgtctgctgcgaggggcccaggaggcgtttcccatggaatatcatcctgctggctatatttacccttgccatggccttca
tggctggaaccattgcaagtttctacagctccaaagccgtgctgatctccatgggaatcaccgccattgtgaccataatagtgaccatcttctgcttcca
gactaaggtggattttacgtcctgcgcagggttgtttgctgttttgggaatcgtcatgttcgtgacaggaatcgttacagccatcgtcttggcctttaaa
tatgtgtactggctacacatgctgtatgctgcactcggagcaatcgtcttcactctgttccttgcgtttgacactcagctggtgattggaaaccggaagc
acactatcaaccccgaggagtatgtctatggcgccctgaagatttacaccgacattgtgtacatcttcctgaacctgctgcagattatcggcagccggac
gtaaacacgagcaaatttcttgggaatgataacaccccccctccctcctcctgtctctcttatggataatgcctccccctttacctctagtctaactttg
ccttagctgagggatctgacatcttcagaggtacccccatttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: