XB-CLONE-3560716
Clone Name: IMAGE:7711727
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Gene:
slc25a20
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968108 [+]
tttactgctgctaaaatgcatgtataaaatgtatccgttagagcgacacagactgtcccaacaagtggtctgtgagccgattaagcgccccattggctac
acagagcgctggtgtgaggaacagtaaaaccctgttacacatataattattatacagagtatttgatacttttagccatatacattcatatactgaagag
gcttatcttccccaaatgactggggcactagcatgtacaccaaaagctgggtagggctatggggtgtctcagtctcagatgtaagacttcggtgttaaga
gaacagcactaatttagcaccgctcactaataaggcaatttattgtgtatttcagtccatgtagtcatggtgctctctgagaggtacctagcagcgcagt
caccgacagccacatcttcacttctacacctaattctgggtttcacatgttcggtgccatccagttcaggaatttcatggctacctcaaatccaagaaaa
catgccgcattagccgggaatgcccgcagcataactgccgtgaagcctttgtatagggatcctatgccttcctctcggatcaactctcgtaaaacgtccc
tgaatccatttggatacttccctgctggagctgtctggaagcgagattttagcacgtctggaggaattgcaacagcccagttaaagattcctgccattcc
gccagcaaagagaattttcggaacactcagctcacttacactgtgtccctctggtgtgaggatgttc
3' EST: CX968107 [+]aaccgcagcctattagtcccatgaagaacttctttgccggcgggttcggagggatctgcctggtgtttgccgggcacccgctggacactattaaggttcg
gattcaaacccaaccaaagccggtgccggggatacctcccttatattctggaacctttgattgctttaaaaagactcttgtaaatgagggtctccgcggc
ttgtacaaaggcatggcagctcccattatcggtgtaacacccatgtttgctgtttgctttttcggctttggccttggtaaaaagctccagcaaaagcacc
ctgaggacattctgacatacccacaactctttgctgccgggatgctgtcgggagtatttactactgcgatcatggctccaggtgagcgaatcaagtgcct
cttgcagatccaggctgcctctggagaagtcaaatatgctggccccatggattgtgccaagcagctgtacagagaggccggaattcggggaatatacaaa
gggacggtgctcacacttatgagagatgttccggccagtggaatgtacttcatgacctacgaatggctgaagaacatcctcacaccagagggacacagtg
taagtgagctgagtgttccgaaaattctctttgctggcggaatggcaggaatctttaactgggctgttgcaattcctccagacgtgctaaaatctcgctt
ccagacagctccagcagggaagtatccaaatggattcagggacgttttacgagagttgatccgagaggaaggcataggatccctatacaaaggcttcacg
gcagttatgctgcgggcattcccggctatgcggcatgttttcttgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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