XB-CLONE-3560653
Clone Name: IMAGE:7711543
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Gene:
plcg2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968151 [+]
tcccttattcttcagccattacttaggcatagtctacagagggtaattcccagtggtgtaaatattcagtggcaacccctaactgctccactatctagca
taccttacttcctgccaccttttgggtaggagagtggccaggaaggggggtctctagagttatggaggaagcataccttgcagttcaggccccccacaac
catggggtctgctttctatatttactgtattagtaataccactgaagactgagttacctaatatgacattacagcaccctctgcttccataatggacaca
tttgtgctaatatattatactgatcaatatcaacatagtaataactggaagcaagtggctctgaaaaattttaactcgctgacattagatgttcagaata
gctccacattggatataggagatgccaagtgcactgctaatatggaggtgatgcttgttgtgatgacaacgggtgcatctagcactgcctagagggcatg
gggatttctctctggctggtaaaatataaatgctaggtgaagcgatttagctggtgctggcatccccctttactgctgtactctgtgccataattagctg
taaatctgggccttaaaactgtcatatttgagttttgttgatcctataacactttattaaattccttaagcgtgaattaaacaattttgaataagacatt
tggatatattgcaacatttatagagcacactcattgttacacaaactagaattgcatgttgatttcatgtgatanaaagcaaacgcacacagaggcatgg
gtaaactggccaaacatttgtataattaacagtctacaataacattaaaatgagaactgncatagatatcttgtccatgccacataaa
3' EST: CX968150 [+]aagttacgtgtccggctataaaatcggctaagccaagcattgttaacagcttatcataaaatgttcactattggaacaatgtttgtattcatccaaataa
ccctgtacattgcccaatctactgaactgagatgtatttatttccatcaacaaccaacaggataactttggaaagtgcaaactttgaaaagcacccaatt
caatggaatccaacaattgttcatgagtatacctggaataagtgtcagttgtggatctatcctttgaagaaacagctctagaagcaccacggacagccag
agagtatcatacaactcagtctttattcatatcctttaaaacccaggacgcctgacgcgtttcgtgcgcatgcgcacttaatcataggcatcagttgtgg
atctaaatgataatgaaaataatacatttgctggtctgcattctgatgccattctgctaacctggacatactgtatcactgacattacaaagagggagaa
atcatgatggtagtatgtgggggagaattagcattgctttatataagtggtgcagtcaaatgacactttgctggagtctttacctgccacccaacccaac
ccacacaatacctcaacaggtaaagttgccatttgccttattaagggagggataaaggatttttctgggttgccatttgctgttggttttacggttactt
cctcacccaatgcacgtagggtagggaataaaggtaaccagaatactaggggggcacatgtgtcacggcagtttagtagaatgngatatagacttattac
acataaaaaaaaatattttgggcgctctgtatgtgctctctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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