XB-CLONE-3560638
Clone Name: IMAGE:7711401
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Gene:
cpa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968161 [+]
ccgaattttccctagtgatgaggctcaggtgaacctggtgagggcgctggaagatgaggagtatttacagctcgatttttggacaagcccctcaagagtt
cttttacccattgatatcagagttccactccaccatctgcaatcagtgaaagtctacctggagtccaacagtatccagtacagcatcatgattcacgatg
tacagaacttgctggatgaagagcaacgcgatatgagacgtaatcgtgcctctgaggatggaaggtctactaacagcttcaattatgctgcctatcatac
atttgatgagatcaacgcctttatcgacaacttggttagtgaaaatccaaatttggttagcaaaatacagatcggttcaagttatgaaggccgtcctctt
aatgttctgaagttcagcacaggtcccaaccgcccagctttctggattgacactggtatccattcccgtgaatgggtcactcaggccagtgctgtctggt
ttgccaaaaagattgtcacagattttggtaaagatccatccttgacaaatacactgaaccaaatggatatcctcatcgaaattgtgaccaaccctgatgg
ctttgtttacactcataaatcggatcgtatgtggcgtaaaaccagatctccaaattctggctcactctgtgttggaactgaccccaacagaaactgggat
gcaggttttggaggtcctggctctagctcaaatccctgcactgaaacctacagaggacgtgctgctcattctgaacctgaagtgaaagccattgtggact
ttgtgaaaagccatggcaacatcaaaggctttgtctccattcacagctactcccagatgcttctcttcccatac
3' EST: CX968160 [+]gtgaaaaaaacactttatttactccaaaaggtgtaaatgttgtttaataaaggttatcacgggtatgctcaataagtatagtgagtgcaagccatgtctc
ctctgcagttggaatgatttggttagctggtagtgcaaatccatagcgcccagtatcacgcagctcaaagctgtaggaatgcttaattccttgattgtag
gtccagtcaatggttcccccgcttgcttggtagatggtactgatgatgctgccataggtgtaagctgtaccgtacagtgaagccaaggcagtaatagctt
tctttgacacagcattcagctcatcgtggtcgggtactcgggtattagtgtatccgtatgggaagagaagcatctgggagtagctgtgaatggagacaaa
gcctttgatgttgccatggcttttcacaaagtccacaatggctttcacttcaggttcagaatgagcagcacgtcctctgtaggtttcagtgcagggattt
gagctagagccaggacctccaaaacctgcatcccagtttctgttggggtcagttccaacacagagtgagccagaatttggagatctggttttacgccaca
tacgatccgatttatgagtgtaaacaaagccatcagggttggtcacaatttcgatgaggatatccatttggttcagtgtatttgtcaaggatggatcttt
accaaaatctgtgacaatctttttggcaaaccagacagcactggcctgagtgacccattcacgggaatggataccagtgtcaatccagaaagctgggcgg
gttgggaactgtgctgaactt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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