XB-CLONE-3560606
Clone Name: IMAGE:7711643
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Gene:
fam50a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968185 [+]
agagagagacagagagagaggcagagtgagacagagagagagagacagacagagagagagagacagagagagaggggcaaacatggcgcagtacaagggg
gctgccagtgaggccgggagggccatgcagcttatgaagaaacgggagaagcagcgggagcagctggagcaaatgaagcagaaaatagcggaggagaatg
tggttaaggcaaatataaataagaaattctctgcacattatgatgcggtggaggcggagcttaaatcgagcactgtgggattggttacccttaatgacat
gaaagccaaacaagaggctctggtgaaagagagagagaagcaacttgccaagaaggagcaattcaaggacctccagttaaagctggagaagcagagggag
agggagagaaagaaagagcagaagcgcaaaatagccagcttgtccttcaacttagaggaagatgaggaatgtgaagatgaggaggctgacgatgactatg
atgatgaggatgatgatgatgaggatgatgatgacgatgcagagaaggaagaactgcccaagaagaagaaaaagctcggcaaaaatcctgatgtggatac
aagtttcctgccagacagagaccgcgaggaagaggagaaccgactgcgagaagaactgagacaggagtgggagcgcaagcaggagaagataaaaagtgaa
gaaattgaaatcacttttagctactgggatggctcggggcaccgaaggacccgtaagatgaagaaagggaacagtatccagcaattcctgcagaaagcac
tggagatcctacggaaagactttagtgagctccggtctgcaagcgt
3' EST: CX968184 [+]ttacacacaggattcccatgacacaacaaggaatatagagtccagttcaggcagcagcaggaacactgtgggtgcaggacaaatggggcagctccagtct
attccttccaggagtctcaccggatcgtatatttatcccatttcttttcggggtcgtacggttcccaccggctggccgggaatatgtgcttgttcttctc
ataccagctccgcaggaccaccttcccagcgtgggactcatccttctctacggtggcgtcgctcagcagtcgcacatcctcgtggacatcaaagttaaag
aggggaccacttttgcctctcgcctttgtcacaatgaagtcataaaaactgtgatggtgcggaatgatgagatcttccttaatgtacatgagctgttcca
cgcctgcagaccggagctcactaaagtctttccgtaggatctccagtgctttctgcaggaattgctggatactgttccctttcttcatctttacggtcct
tcggtgccccgagccatcccagtagctaaaagtgatttcaatttcttcactttttatcttctcctgcttgcgctcccactcctgtctcagttcttctcgc
agtcggttctcctcttcctcgcggtctctgtctggcaggaaacttgtatccacatcaggatttttgccgagctttttcttcttcttgggcagttcttcct
tctctgcatcgtcatcatcatcctcatcatcatcatcctcatcatcatagtcatcgtcagcctcctcatcttcacattcctcatcttcctctaagttgaa
ggacaagctggctattttgcgcttctgctctttctttctctccctctccctctgcttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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