XB-CLONE-3560556
Clone Name: IMAGE:7711505
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Gene:
cd74
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968222 [+]
tatggcctatttcatcacccagcagaattccaagattcagaaacttgaccaaaccaccaaacacttgcagctgaaagatatgatgaagaatcttccagga
tcgcctcctgcccaaattaaatcaaagatgagaaccttcaatatccccatggcgttaaaactatatgatggctcagaaacaaatatgaatgaactggagc
atctggctcagatcgataacaaagtggaagatgctgcaaaatacatgttgctgagaggaaaccctctaaggaagtacactttaccaaatggcactatcct
ggaaaacctgagagagctgaagaagtccttgactgaccaggaatggatgaactttgatgcatggatgcatcagtggtatctcttcttcttggtgcaaaac
actggaaaggcagctgaacccctcccaccgcaaaagaatattgcagtcacaggtgcccccctgatgactgagtgtcagacattatcaaggatccccaccc
tgactggcgcttataagcctcagtgtgagcaaaatggtgactttaagccgaggcaatgctggccaagcactggctactgctggtgtgtttaccgtaatgg
aactgaggtccctgactccagaacccggtggtcaagacctgcctgcagtgatgtcatggagccagaagaaccaatttttctgggatcaacacccagcgat
gactttaatcccattgattaaagcatcggatcagcacgtaacctcctctgcagtatctaccctgattcatacaagcagcacaagaggattcctattaatc
tgactttatgttgntactaattgctatggcattctggcctcaggtttctcaataggcttaaggtag
3' EST: CX968221 [+]caagatgtaactgctcattttattaggcatggaataaggtatgtgcacgttatacagaacctgtaagtctgacagaagccgcaattaacagcagagaaaa
agttaaaaaaaaaataaaaatggcagccatatttctatcaaaacacaattagcgataaatgaatgaacaacgattttatcagtgcccatttgaattgtca
aatcatgttaatgttatcacattccccagaccgtaccttactgagacctccttaaccatacagaaactactagatttgttagtagtggcaagacgtggag
gatttgtccatggtatagagacatagacaagccacttttcagaagtactagttagtcagcctcctggtagatacttcctttcaatcatgtgcagctacaa
tcacagtgggactgatctgtgtaaccataccacttattattcctttaacatgctgctgtaacacacactgtaattcataaaaacaaacaaagtactaaac
actaccttaagcctattgagaaacctgaggccagaatgccatagcaattagtaacaacataaagtcagattaataggaatcctcttgtgctgcttgtatg
aatcagggtagatactgcagaggaggttacgtgctgatccgatgctttaatcaatgggattaaagtcatcgctgggtgttgatcccagaaaaattggttc
ttctggctccatgacatcactgcaggcaggtcttgaccaccgggttctggagtcagggacctcagttccattacggtaaacacaccagcagtagccagtg
cttggccagcattgcctcggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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