XB-CLONE-3560408
Clone Name: IMAGE:7711520
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Gene:
tbc1d31
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968327
[+]
attagctcctccctgtttaatgcattggtgtttcagtacatcagcagggctgtgcgactttttgtattgaaaggatccctgggaattccccatgcgagga
agaaggggcgatttatttggatttgtcctattgtcacccttgtgcatcattcatatagcagccgcctgtgagagagagcgttatgggtaatctgcagcga
acagtagggtgttcccagtgcaggcaggctcctcactgcaggtacctcttgcaataagtggccccgcagttacctggttgtgtgaaaaaatatttatgga
aatgcgagagtgttagggcaatatattccagtttcaaagatggcgctgaagggatgtccgtgtagaacgcccctggcggctatagcggtagttgtaggca
ccactcataatataggggactacattacccataatcccatgggtcagtcggaatacgcttcctggatactgggctgatgttatgtagcaacatgatgaag
gaaacataaaaggcgtttttcggtagcccccttgctctttccgagctcatcatcattactatgctgatatattagatctcatcttggatctaggaccaac
tgcaacacttagcaaatctggcaccatgcaaaccacagacttgggcaacaaagaatctggaaagatttggcacaggaagcctaacccatccaccaacgag
gggatcatagtgaatattgtgcacgttgatgcatcccacccctccaagtctgcacgatttctccatgctggatttgacagcgctggggactccttcatag
caggagatcacctaggaaatgtctatctgttcaacttaaacagaaaacagtttgatcttgtgcagaaacaatgcaggcatgtacagcaatagc
3' EST: CX968326
[+]agggcgagaggcttacaatgtgaattaaatgcactgctcactagtttaggtccagtttgtttcagagaagtgcaactgagggtattttctgcgtgcttgg
gtagcaagtttctgtcggagctctcgtacttctgatatcagggctcgctctctgttctccagctcccctcttcctctgtctaaagaaaagtgagtggagg
agtcacttgcagagacatcgtttaaacaaacattttcggctacgtgccgctcagcgctgctactgaaatcattctgtgtggaattgcggcttaaatcgcg
aagtctctcaaaatattcatttccatcctctgagatattaacaggttcagcttcctgccttaacctctcctgtaagaactgattccttttggcccgcagc
gctgagcttgcaagctcctctgtttccagttgagtcattttctcagagacctctgcccactttcttgtttccatggctttcagaacatcacataactctc
gctctttctctcggacatcgtcagtcattttggacacttctacgtagcgcttccctcgctcctggtcatcacatttctgtttctgaagggtttgtatttt
ccagtcggtttccacacacagacgttcagcctttgcaaggctctcttcaagcagcttcttatctttaaggtcttcctctgtttccataagcctccaaaac
atttgttcttccaggtcggcccttctccggttggcatccatctctcctttgacttcctgggtcacagcctcctgctccttagcaagttgctgttcaaaaa
atctcctttgtgcttcaagctccatctgcctgatttcaacgtctttcgctatggtggcagtttcccgttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: