XB-CLONE-3560325
Clone Name: IMAGE:7711624
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968384 [+]
aaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctacaaatgtggtggtatcgacaagag
aaccatcgaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggt
atcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatca
ctggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgc
cctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaatc
gttaaggaagtcagcacatacatcaagaagattggttacaaccctgatactgttgcctttgtacctatttctggatggaacggtgacaacatgcttgagc
ccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctggaagctcttgactgcattttgcc
accaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtaccagttggtcgtgtggagactggt
gtcctttagccaggcatggtggttacttttgcccctgttaatgtaacaact
3' EST: CX968383 [+]ctcctatagggcgagaggcttttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaaaaactgccacaaacaaatgtgtcc
acgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaattgatagaaagagttctg
agaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactttgccagc
tccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtcagagaag
ctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttaccagaacggc
gatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctggcctgggtggtt
caggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtccttcacagaaacg
tttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgttacattaacagggg
caaaagtaaccaccatgcctggcttaaggacaccagtctccacacgaccaactggtacagtaccaatac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: