XB-CLONE-3560296
Clone Name: IMAGE:7711532
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Gene:
slc22a4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968407 [+]
catcgattcaattcggccgaggcaatttcaatagctgttaatctgacttaagaagtcagaaatctctttggaagttactactttggaagtttttcaagtt
ttattggacttggaattacagtggatttaagtttgttttcttgacactacagaaaaagactaatgtcagagtgaaatgagagtagtgatttgaaataatt
acaatgaatacaacaacagatataaccaattgcattactgatgctcccatggcagcaattccagcctggcaaaactgcttatactctcagattgaactgg
ggccatgagggaacagccctttttcagccataaattcctctgttggattgagatttggagtaccctggaggatgcagatatttttaagccattcttgtag
ttttgcctttatgcttactgaaatacacttcttttcccaaggcttacacttcttgcaggatttcactgtgtgttgctacactgattctaccctcttttca
cacgagttttccaacacctgctggtgtttagtctaatggccaaaaggctcagttgttagattcattagactatagaaccttcttccagttgatttctgag
tctccctcttgccttctggcaaattgtaagcaaaatgtcattattttactcaacagggcctttctattttgccactcttactgagtttgcacctatgcaa
cagttgttatatgaaaagtctctccaatctttagtcttcaaactccctcagagttgttatcggtctcttggtggactccctcacagtcttctttttccat
agtcacccaccttgggtgaagatctcttnctggctgatttacagctatcctgtaccctttaatttnctatgattcatatagcaaaactcc
3' EST: CX968406 [+]atatttcaataactttgaatttttttatttataaaaatgattacaaatttcagaatttacaatatatgtaagttttttttcacaacagtttcccttttaa
taaaaaaactacatgtgcactcaaaaaccaaatgaaattatgaatttatatgggtaagccacagcaaccaataaatgtctgcttattccaacttaattag
atcagtacaagctaactgcttattgggtgctgggtgaggcaaactttgccccaagtagtaaatataccctcccaataaatctaggaaatagtgagaaact
gtatgcaaatatcaccatgggtctcatactacaacacagacactttcatatcaaagtgagaaaagatctctgtaatgcaagttaaattaatcacaatgga
aagaagcaaaataacataccaccccacagacacaccaaaaccatcaccattggtgcagccaactgttccccaaacacagttagatgaaaattacctgtgt
ggagtcaaaggcttcaaggaattgaagcatgaacatgtgtatcaagttcaccttctagtaagccagtattatggcataacttacactatgtaggtccaga
aaagggtaactgtaaagctaaagttaggggattgataaaaggaaatttctaaatcactgaatatcacttggagttttgctatatgaatcataagaaatta
aagggtacaggatagctgtaaatcagccaagaagagatcttcacccaaggtgggtgactatggaaaaagaagactgtgagggagtccaccaagagaccga
taacaactctgagggagtttgaagactaaaagatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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