XB-CLONE-3560005
Clone Name: IMAGE:7711418
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Gene:
tmem79
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968612 [+]
aacacaaggcactgctataaatacagtgagaattactaagaacattgtgtaagaagatgggggacaagtaatggggtaagtgaaattgttcttttctgcc
gtatcatgaaatcatatagttgaacccacgggtctctttactttcccttagtacccaccctggtgcagagctctaaatctctatgtgctgctctctttcc
cattgtcagccagggggcaactccatgttcctgggaacccccagttaagaaaaaaaaaacatttgttctttgcggccctacccatgtgacctgatcagcc
tcccagcctccgtgttcaatgtgcttcctatgtccttaatggacctccagctaattgtagctcaatgcccacttacttttgtggctccactacatactct
attgcactgattaaccaaccaatcaaaaaacaggtttaatagaagaaatgttgtataaaggtggtaacattgacctgagtaagaaacatccatgatgcat
cagtaagcccaccctgggttcctgtgtttataaccgattaccctgaagggacgtaaagttctagagtgttgagctgaggctgctcttttgcctctttgtt
ctagatttagttcttttcctctctgtggccttcaacccaaagtgtctacttttgacttggagtcctccttgggaggtaagcccaaatacagcatcctctc
cgggtccacaaggaacatgaagcagaggttacaagccagcatgcagaccaacggcaggaagggcaggccgtagccaaagccacggaatgagcggccaacc
gcaaacgacagccag
3' EST: CX968611 [+]cccggaccacatcacgaggctaagcaacgccggactgtttgatccctgtgaggaccaagttgaaaagagagaagcagaagacctaaaaggagaagaggac
tgtgactcgcaaacggtgaaccctgccttctttatccaacacgagaggctagaaagcttttctgggagtagccacagaaaggtgcagctggaaatgtgcg
aacatttggactctgatagaggagaaccagaggcagaacctctactccgttctcaccattccaggtcagtagatgtggagtccatgcaaggaggacctgt
tggctgccggtgtgacaagaactatctcaagatcgtcggctccttcttcctctctctctttgtgttcccggccttcctgtgtttggcttacacattcctg
ccctttgatgccccgctgatgccagatatcaccaccaggcttatttacaccttacggtgtggggcatttgcctcatttcccatcatcctcggagtaatca
tccacgggatctcgcggctctgcacctcttccttcgacccctttaagcccaaggtgcgggaggtggccattcaccggcggtttgtcaagcagagcacctt
cctgttcgttttgtatttctttaacctgtgcgtgttggtgacttacctgccccagaactacctgaagctcatcccgctgctcacctgtctctttgcactc
tcgcagctgatatactggctgtcgtttgcggttggccgctcattccgtggctttggctacggcctggccttcctgccgttggtctgcatgctggcttgta
acctctgnctcatgttcctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: