XB-CLONE-3559912
Clone Name: IMAGE:7712097
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Gene:
glul
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968680 [+]
catgtccgtctcccacagttcgagactcaacaagggggtgagggaacagtacatgaaactgccccaaggagggaaggtccaggtcacctacgtctggatc
gacggcaccggggagggagtgaggtgcaagacccggactctggatcaggaacccaaaaccatagacgaaatcccagaatggaacttcgatggatccagta
cgtaccaagcagaaggttcaaacagtgacatgtatctcgtcccagtccagatgttcagagaccccttctgcctggaccccaataagctggttatgtgcga
agtcttaaaatacaaccgcaagtctgcagagaccaacctccgacacacgtgtaagaagatcatggaaatggtgactgaccatcgcccatggtttggaatg
gaacaggaatacaccctgctgggcattaacgggcacccgtatggttggccagagaacggcttccctgggccacaaggtccctattactgcggcgtggggg
cggataaggtgtacgggcgggacatcgtggaggcgcactacaaggcgtgtctatacgccggcatcaagatctgtggcaccaacgcggaagtcatgccctc
ccagtgggagttccaggtgggaccgtgcgaaggcatcgacatgggggaccacctatggatggccaggttcatcctccaccgggtgtgcgaggacttcggg
gtggtggccacgctggaccccaagcccatgaccggaaactggaacggcgctggttgccataccaactacagtacagagagcatgagggtc
3' EST: CX968679 [+]agccaattaacaatcccgccctccccaaagggagaaactgaaggaatcagtcggcaccagccaatcagattccagcagggtcactcgctttgttcttatc
ctatagatctaaaataatcagcagccccccccaaccttacagacaatatggtagaaccatctatacaccttagaagaattcaagtagtgtcgggagtcta
acgggttaaaaaaacatggcggcgtctcgggagatgccatagtgattgaccaggagaagccagctcccagctcaggtctaatagtctttggtctcgctgc
cggtctcgttcaagatggtcgttcggaccagcgcctcggtaacggcgtaggggtcgcagtttgctgccggccggcggtcctcgaagtaaccgtagccttc
ctgccccacctggcgggggatccggatgctggcaccccggttggcgactccggccgagaactcgtggatactggacgtctcgtgctgcccggtgagtctc
cgggagttgtccttcccgccccgggggtcatacacgcagatgtggtagttgtgcctcttcccgagcttctcgatggcatcttctatgtgtttgagtcccc
cttcgaccctcatgctctctgtactgtagttggtatggcaaccagcgccgttccagtttccggtcatgggcttggggtccagcgtggccaccaccccgaa
gtcctcgcacacccggtggaggatgaacctggccatccataggtggtcccccatgtcgatgccttcgcacggtccccacctggactcccactgggagggc
atgacttccgcgttggtgccacagatcttgatgcccgcgtatagacacgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: