XB-CLONE-3559876
Clone Name: IMAGE:7712005
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Gene:
gapdh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968705 [+]
cgcctggtgacccgcgctgctgttttaagcggcaaatgcgaagtcgttgcaatcaatgaccccttcattgacttggactacatggcttacatgttcaaat
atgactccacccacggccgattcaagggaacagtgtgtgttgagaatggcaagctggttatcaatggcaaggcagtcaccgtcttccaagagcgtgaccc
ctccaacattaagtggggcgatgctggtgcagtgtatgtggtggaatctactggagtcttcacaaccaaagagaaggccggtttgcacttgaagggaggt
gccaagcgtgtcattatctctgccccctcagctgatgcccccatgtttgttgttggggtgaaccatgacaaatatgataactctcttcacgttgtcagca
acgcatcctgcacaaccaactgcctggctcctcttgcaaaggtcatcaatgacaactttggcatcctggagggactgatgactaccgtccatgccttcac
tgccacccagaagactgtggatggcccatctggcaagctgtggagagatggcagaggtgccggtcagaacatcatccctgcctcaactggtgcagcaaag
gctgttgggaaagtcatccctgagctgaatggcaaactgacaggaatggctttccgtgtccccaccccaaatgtctccgttgtggatctgacctgccgtc
tgtccaagcctgccaagtatgatgacatcaaggctgccattaagactgcagcacatggcccaatgaanggaatcctggaatacacagaagaccaggttgt
ctccactgactt
3' EST: CX968704 [+]aaactaccaggatgggattgtgactctgggccccttcagtgacagagaggggttggtggggcgacaggtaagtgcttattccttagaggccatgtgacac
atgagatccacaacacggtggctgtagccacactcgttatcataccaggaaaccagcttcacaaagttatcgttcagtgcaattccagctccagcatcaa
agatggaggaatgagtgtcaccattgaagtcagtggagacaacctggtcttctgtgtattccaggattcccttcattgggccatgtgctgcagtcttaat
ggcagccttgatgtcatcatacttggcaggcttggacagacggcaggtcagatccacaacggagacatttggggtggggacacggaaagccattcctgtc
agtttgccattcagctcagggatgactttcccaacagcctttgctgcaccagttgaggcagggatgatgttctgaccggcacctctgccatctctccaca
gcttgccagatgggccatccacagtcttctgggtggcagtgaaggcatggacggtagtcatcagtccctccaggatgccaaagttgtcattgatgacctt
tgcaagaggagccaggcagttggttgtgcaggatgcgttgctgacaacgtgaagagagttatcatatttgtcatggttcaccccaacaacaaacatgggg
gcatcagctgagggggcagagataatgacacgcttggcacc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: