XB-CLONE-3559816
Clone Name: IMAGE:7711915
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Gene:
eef1akmt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968749 [+]
cagacgtggcgtagggttggatattttcggcaagcagaaaaacgcttgccgaaaattccgccctacacctcctacttgtgcttgcacctgaatgaatgga
atacgctcgggtacaggcacatgtagcggaaatacgcataaaaacatgagagtttgaaagcctccagtttttatgagtatttcggcaatttgcagttggt
tttcattttttatatgttgtcttcgtagcttttagttcagcagctctcctgtttggaatttcagcagctatctggttgctagggtcttgtttaccagcga
ccaggcagtggggtaaatgagagactggaatatgaatagaagagggactatataaatagatagggaataaaaagtaacaataaaacaaagttgtagcctc
aaggagaaaatgttttttggctgccatggtcagtgaaggcaaattatttaaaaaagaaaaactaggacaaataaataatgaagatggattgcaaagttac
tatagtacattcaataacatactaaaagctagtaccacccctttaacataaaatcagtgtagtctgcagcatgtcattaaagggattgttcacctttaag
ctcatttatagaatgtcctgttcttagcaaccttgctattggtattcatatttgatttttatagttttcttatttatttcccttctactgactctttaca
cttttaaatggaggtcactaacccagcaaccaaataaacaattgctctgtaaggctacagttttgttttgtttttgtcaaggctgcagttttgttttttg
tgggctaaggtaaatttgaccctaaca
3' EST: CX968748 [+]caatatgtcattttatttacttacagagacccccaaagccaactctagagggttagccctaggaaacatataatcaccacttaaacctgttactctcggg
gtccgcatccatcctttggtcttccagactgacagctgataaatgttacactgccagttattaatattagcagccatacactacattctaactctctttt
tgtggacaaaaaactcacaaatctgataaagtgtgagaggcaaatgatgagagaggaaggccagcttcatactgcatctgctggctatgtgtcagggata
accctgccagagcaaactgcacagatatgggaagcatcatgccaacctgtccaatgtaagttcataatttgagtagcaccgaaactcattagccaggctt
cgtgtgtgttttgggataaacttgcatattttaaggccaagtatctgagctgccagatcctccatgatagcgcctgtgcacagtatgatcttcccccggg
agaggtacttgatggtctgtgccgtgttccgcaggcactcttctgacaagtacggggggtctgcaatgactatatcaaagctactctgctccaggctctc
cggcagcttcagtggttcattgtaatcatagaagacaaaatcatctccataaattgcaaagcgctggtcatattctaagagctgcacgtgcaagctctct
ctggcaagccctctgaccttctgatagatgctgggggcactgatgcaagcaatcctggaacaaggcaaattaggcagtaaaaataagagga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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