XB-CLONE-3559767
Clone Name: IMAGE:7712063
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Gene:
aagab
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968784 [+]
tattggaaaagatgagtgtccggccaacacacaaggagtgaagcagtacccatggaggatagataacaagtactactgtgctgatgtgaatatctgtgtg
gtgcccagcacaacctgtgtcactgcacagatagcagagactgttcaggctttcattgtctactttgacagcaaaacgaaactgggtctagagaaggtgt
ctgcatggctcccactgttggaagactgggtgctggacgtgatgatcctggtgtgcgaccgtgtgtcagaagctggagtgaatcgccagacagcgcagga
gtggtgcatcaagcacggctttgagttggtggagctgaatcctgatgaactgccagatgaagatgatgatttcccagaatcaactggtgttacccgcatt
gtccaggccttgaatgccaacgtgtggtctaatgtggaaatgaagtgtgaacaagctttcgggatcttcagctcattggctgctgtgaatcaccctccag
agaccagcggcgagctgcagggtgatcaggcgcctgcagccagtgagggaactttggagcagtgcagaggagaggactctggaaacccccgggattcgca
agttgataccatagttgatccaatgctggacctggacatcagagagcttgccagtcttacctcgggggaggnggacatagaaaacttcagccggcttttt
accaaattgcaggagatgaaagataaagctgccactttacctcatgaaca
3' EST: CX968783 [+]ttacatcccnttttaagcaacaaagtgtcaaaaaagacttgttttgtagaggtatgcgtccgtagtgattctttgggattaaaatgcatgtgcctcttgt
aaatatcaaaacctgtagtttaaatgaatataaaatattttatttagatttgtcatcctctaataaatatacaagcaaacaatcaccgacaagtaactac
taagtggaaacatcatgaaggccgaccagcacggcaggtagaagggaggatggaaacgtgagcaagtcaggtcctaatgctccactctcattggaaacag
caggggctcatggctcttagctctcttcctcagatgacagaccttcaatctcatcctggtctcctccaatcgccatccagaaagctttggcaaccttttc
tgcgtgcagttttctctgttcatgaggtaaagtggcagctttatctttcatctcctgcaatttggtaaaaagccggctgaagttttctatgtccccctcc
cccgaggtaagactggcaagctctctgatgtccaggtccagcattggatcaactatggtatcaacttgcgaatcccgggggtttccagagtcctctcctc
tgcactgctccaaagttccctcactggctgcaggcgcctgatcaccctgcagctcgccgctggtctctggagggtgattcacagcagccaatgagctgaa
gatcccgaaagcttgttcacacttcatttccacattagaccacacgttggcattcaaggcctggacaatgcgggtaacaccagttgattctgggaaatca
tcatcttcatctggcagttcatcaggattcagctccaccaactcaaagccgtgcttgatgcaccactcctgcgctgtctggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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