XB-CLONE-3559591
Clone Name: IMAGE:7711937
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968903 [+]
gaagagaatccaccgccatctgccacaatggtgaacttcacggtagaccagattcgttccatcatggacaagaaggcgaacattcgtaatatgtcggtca
ttgcccatgtggaccacggtaaatctactctgactgactcactggtatgcaaggctggtatcattgcttctgctagggctggtgagactcgttttacaga
taccagaaaagatgaacaagagagatgtatcaccatcaaatcaacagcaatctctctcttctatgagctctctgagaacgacttggcttttatcaagcaa
agcaaagatgggtgtggcttccttataaacttgattgactcccctggccatgtagacttttcctctgaagtaactgctgctcttcgtgtcacagatggtg
ccttagttgtagtggactgtgtttcaggcgtctgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaa
caaaatggatcgtgctcttttggagctgcagttggaaccagaggagctctaccaaacttttcagcgcattgtggagaatgttaatgtgattatttccacc
tacggtgaaggagagactggacccatgggaaatatcatgattgaccctgttcttggcacagttggttttggctctggtctgcatggctgggctttcacac
taaaacagtttgcagagatgtatgtcgcaaaatttgcagcaaagggggaaggtcagttggctcctgcagaacgggccaaaaaagtggaggacatgatgaa
gaagctgtggggtgataggtattttgacccctgc
3' EST: CX968902 [+]tttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttacttccc
cagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttccaacag
aatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaaggaagtt
atccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccaggtaga
atttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcaggtatg
ccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacctgctc
agggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatgatctgt
cctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcanattttcttcacacaggacaccctcctttgtag
cccactggaatccctgcacaacgctgtctttgatttcattcagatactgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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