XB-CLONE-3559458
Clone Name: IMAGE:7711808
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Gene:
cfi
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968999 [+]
ccgagggctttttctatattaacacattaacgagctcattttctggggcagaagatgtgagaccgaataaatgtgaattcacttccctctcctgccataa
agtcttctgtgctccgtggcagaggtgtgtggccggggtatgtcgctgtaagctaccatatcagtgcccacggaatgcaactactgaagtctgcacagag
gggaaaaggaaactgcaaacctactgccagcaaaaaagtgttgaatgttcaaatccttcaaatgtcaaatacagattttcctcagaagctccttgcactg
atacattttcagtaagattaacagggagcaaagaaacaagcaaaggaatcataaaagtgaaattacctacactagaaaaggaattgtttatatgtgcaaa
acaatggagtctgagggaagccaatgttgtttgcaaacagcttggatttcccagaggcgctgatgcaccagcatcaaacaaagatcttccattagtaaca
aaggagtccccagaagaacattgccttcaagcaacttgtcgaggactagagaacagcttggccgaatgtgctctaaggaagttacctgtggagaagcagg
ttgctgaagtaaagtgttatacagaaaacagagattgtggctctggtgagtttaaatgttcaaatggcaagtgcattccctcagaactggcctgtgatag
tgaaaatgattgtggagatctcagtgatgaactatgctgcaaagattgcagtgctggcttccattgtaaatctgatacctgcatcccagagaggtataag
tgcaat
3' EST: CX968998 [+]cagcgaacaatatttttattctttgcacgtgcagtaaaaaattatgaacgataatgatgaaatcaatcctcccagtgcatagtagcaaataatgatgaca
gcatctcagacattgtactttgagataagggatcttcccacctgatgtgaaatccattcatagtagtgagcaactttagtgtatacaccagggaaaccag
gaactccacagttttctccccagctaacaatgccccaaacataagcaacgttgtttacatcatagcacaccaagggccccccagagtcacccttgcatgc
atcgatggagccatcataagttcctgcacattccattttgtcaaggaaacgctccttataaacttctgaacaattattcattaggttaatgtggccccat
ttcaggtgaaacaccctggacattcctttttcccgtccccatccagaaactgtgcatgtgtcccctgccctaaactgaaatggtgaccaggggacgcagg
caggcaccatgttattgtcagtctgcatgcactttggattactgtatatgttctttacttccaacaaggcaatgtcattttcatatgtattggggttgta
cgactcatgaactatgacacttttaactgggaaactgtctatgtctttgtcataagacagtcgatccagcagttcaagtatgatgagatatctttggggg
tggttagccctgacacagtgagctgctgtcagtacccagcagcctccaatataaatccctccacagtttacagatgtgccatctttgattgctacttgcc
atggaaactgattctttacagcatttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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