XB-CLONE-3559415
Clone Name: IMAGE:7711908
|
Gene:
fth1.1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX969031 [+]
ggatccatcgattcaattcggccgaggcttttttagaccaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaa
acaccgaaacgccgccatgcaatcccaggtgcgtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcc
tatgtctatctgtccatgtcttactatttcgatcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagc
atgccgaaaagttcctcaaataccaaaacaaacgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctgga
agccatgcaggccgctctgcagctggagaagactgtgaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgac
ttcctggaatctgagtacttggaggaacaggtgaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgcagaatggca
tgggcgagtacctgttcgacaagcacaccctgggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctctgtttcaaaatactgtc
tctttactatctgtgtggattacaccatagctcccatgcccctgttgacacctttttatcttgaggatgatgggaataaatctgtgctattactgttctg
aatcatgcagaagctgctgtgcttgtca
3' EST: CX969030 [+]tgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttattcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagct
atggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctgactggttgtttccaccctgagagtgcgcttagctactctcccccagg
gtgtgcttgtcgaacaggtactcgcccatgccattctgcggcaccccaaggcgcttcaggtttgtgatgtagtctccaagctccttcatggccttcacct
gttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagcttgtgcagatccagtaaagcctggttcacagtctt
ctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttcttgatatcctgaaggacggcgcggcccccacgtttg
ttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctccttgaagaacttggccacatggtggagtgccacgtcatcacgatcga
aatagtaagacatggacagatagacataggaggcatacaactccatgttcaccatacggttgatggcggcttcgcagtcgctgttgaagttctgacgcac
ctgggattgcatggcggcgtttcggtgtttgctggtggcgcaaaaaagtcac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: